Original Article

Journal of Investigative Dermatology (1985) 85, 365–367; doi:10.1111/1523-1747.ep12276992

Ultraviolet Radiation-Induced Histopathologic Changes in the Skin of the Marsupial Monodelphis domestica II. Quantitative Studies of the Photoreactivation of Induced Hyperplasia and Sunburn Cell Formation

Ronald D Ley and Lee A Applegate

Department of Immunology and Cell Biology, Research Division, Lovelace Medical Foundation, Albuquerque, New Mexico, U.S.A.

Received 7 January 1985; Accepted 21 May 1985.

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Abstract

Induction of sunburn cells (SBCs) and hyperplasia of the epidermis of the marsupial Monodelphis domestica by ultraviolet radiation (UVR) has been studied. A dose of 500 J/m2 (approx1 minimal erythemal dose) from an FS-40 sunlamp induced measurable numbers of SBCs with a peak number at 32–48 h post-UVR. Post-UVR exposure of skin to photoreactivating light suppressed the induction of SBCs by approx75%. Pre-UVR exposure to photoreactivating light had no effect on the induction of SBCs. Induction of hyperplasia also was suppressed to a similar extent by post-UVR photoreactivation treatment. These studies identify pyrimidine dimers in DNA as the major photoproduct involved in the induction of SBCs and hyperplasia in M. domestica by UVR.

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