Original Article

Journal of Investigative Dermatology (1985) 85, 203–206; doi:10.1111/1523-1747.ep12276675

Expression of Activation Antigens by T Cells Infiltrating Basal Cell Carcinomas

Francisco J Guillén1, Calvin L Day Jr2 and George F Murphy3

  1. 1Visiting Fulbright Scholar, Department of Pathology, University of Alicante, Alicante, Spain
  2. 2South Texas Medical Center, 7711 Louis Pasteur Drive, San Antonio, Texas, 78229
  3. 3Department of Pathology, Brigham and Women's Hospital, and Dermatopathology Unit, Harvard Medical School, Boston, Massachusetts, U.S.A.
  4. 4The Chemosurgery Unit, New York University Medical Center, New York, New York, U.S.A.

Received 14 January 1985; Accepted 2 April 1985.

Top

Abstract

The association of T lymphocytes and dendritic cells with the stromal mononuclear cell response to basal cell carcinomas has led to speculation that cellular immunity may, in part, regulate the growth and development of this neoplasm. It has not been established, however, whether these T cells are functionally competent, or simply coincidental bystanders. We examined the immunologic phenotypes of mononuclear cells in 32 lesions of basal cell carcinoma obtained from 26 patients. The majority of infiltrating mononuclear cells were T cells that were equally distributed between the helper/inducer (Leu 3a+) and cytotoxic/suppressor (Leu 2a+) sub-types; a minority of cells were dendritic and expressed Leu 6 antigen. Virtually all T cells and dendritic cells were HLA-DR+, and many (>30%) of the T cells expressed antigens consistent with stages of ongoing activation (T9, T10). TS2/7, a novel monoclonal antibody recently documented to identify activation-specific sub-components of 210/165/130 kD glycoprotein complex present on the surface of mitogen- or alloantigen-stimulated human T cells, was also used. Greater than 50% of the T cells observed were TS2/7+ These observations provide in situ immunomorphologic evidence of stromal T cell activation in association with basal cell carcinomas, and suggest a role for active and ongoing cellular immune mechanisms as a determinant of local biological behavior of this neoplasm.

Top

References

  1. Murphy, GF: Cell membrane glycoproteins and Langerhans cells. Human Pathol 1985 16: 103–111,  | ChemPort |
  2. Murphy, GF: Immunodiagnosis in dermatology. Arch Dermatol 1985 121: 731–733,  | Article | PubMed | ChemPort |
  3. Bhan, AK, Mihm, MC Jr, Dvorak, HF: T cell subsets in allograft rejection. In situ characterization of T cell subsets in human skin allografts by the use of monoclonal antibodies. J Immunol 1982 129: 1578–1583,  | PubMed | ChemPort |
  4. Ruiter, DJ, Bhan, AK, Harrist, TJ, Sober, AJ, Mihm, MC Jr: Major histocompatibility antigens and mononuclear inflammatory infiltrate in benign nevomelanocytic proliferations and malignant melanoma. J Immunol 1982 129: 2808–2815,  | PubMed | ISI | ChemPort |
  5. Bhan, AK, Harrist, TJ, Murphy, GF, Mihm, MC Jr: T cell subset populations in lichen planus: in situ characterization using monoclonal anti-T cell antibodies. Br J Dermatol 1981 105: 617–622,  | PubMed | ChemPort |
  6. Cotner, T, Williams, JM, Christenson, L, Shapiro, HM, Strom, TB, Strominger, JL: Simultaneous flow cytometric analysis of human T cell activation, antigen expression and DNA content. J Exp Med 1983 157: 461–472,  | Article | PubMed | ChemPort |
  7. Hercend, T, Ritz, J, Schlossman, SF, Reinherz, EL: Comparative expression of T9, T10 and Ia antigens on activated human T cell subsets. Hum Immunol 1981 3: 247–259,  | Article | PubMed | ChemPort |
  8. Reinherz, EL, Kung, PC, Pesando, JM, Ritz, J, Goldstein, G, Schlossman, SF: Ia determinants on human T cell subsets defined by monoclonal antibody. Activation stimuli required for expression. J Exp Med 1979 150: 1472–1482,  | Article | PubMed | ISI | ChemPort |
  9. Ko, HS, Fu, SM, Winchester, RJ, Yu, DTY, Kunkel, HG: Ia determinants on stimulated human T lymphocytes. Occurrence on mitogen and antigen-activated T cells. J Exp Med 1979 150: 246–255,  | Article | PubMed | ISI | ChemPort |
  10. Hemler, ME, Sanchez-Madrid, F, Flotte, TJ, Krensky, AM, Burakoff, SJ, Bhan, AK, Springer, TA, Strominger, JL: Glycoproteins of 210,000 and 130,000 M.W. on activated T cells: cell distribution and antigenic relation to components on resting cells and T lines. J Immunol 1984 132: 3011–3018,  | PubMed | ISI | ChemPort |
  11. Eaglstein, NF, Hernandez, AD, Allen, JE II: Lymphocytic response to basal-cell carcinoma: in situ identification of functional subsets using monoclonal antibodies. J Dermatol Surg Oncol 1982 8: 943–947,  | PubMed | ISI | ChemPort |
  12. Murphy, GF, Krusinski, PA, Myzak, LA, Ershler, WB: Local immune response in basal cell carcinoma: characterization by transmission electron microscopy and monoclonal anti-T6 antibody. J Am Acad Dermatol 1983 8: 477–485,  | PubMed | ChemPort |
  13. Claudy, AL, Viac, J, Alario, A, Thivolet, J: Identification of mononuclear cells infiltrating basal cell carcinoma. Acta Derm Venereol (Stockh) 1981 61: 555–577,  | ChemPort |
  14. Macadam, RF: An electron-microscopic study of basal cell carcinoma. J Pathol 1978 126: 149–156,  | Article | PubMed | ChemPort |
  15. Haynes, BF, Hemler, M, Cotner, T, Mann, DL, Eisenbarth, GS, Strominger, JL, Fauci, AS: Characterization of a monoclonal antibody (5E9) that defines a human cell surface antigen of cell activation. J Immunol 1981 127: 347–351,  | PubMed | ISI | ChemPort |
  16. Sutherland, R, Delia, D, Scheneider, C, Newman, R, Kemshead, J, Greaves, M: Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin. Proc Natl Acad Sci USA 1981 78: 4515–4519,  | Article | PubMed | ChemPort |
  17. Cotner, T, Mashimo, H, Kung, PC, Golstein, G, Strominger, JL: Human T cell surface antigens bearing a structural relationship to HLA antigens. Proc Natl Acad Sci USA 1981 78: 3858–3862,  | Article | PubMed | ChemPort |
  18. Hemler, ME, Ware, CF, Strominger, JL: Characterization of a novel differentiation antigen complex recognized by a monoclonal antibody (A-1A5): unique activation-specific molecular forms on stimulated T cells. J Immunol 1983 131: 334–340,  | PubMed | ChemPort |
  19. Murphy, GF, Bhan, AK, Sato, S, Harrist, TJ, Mihm, MC Jr: Characterization of Langerhans cells by the use of monoclonal antibodies. Lab Invest 1981 45: 465–468,  | PubMed | ChemPort |
  20. Murphy, GF, Bhan, AK, Harrist, TJ, Mihm, MC Jr: In situ identification of T6-positive cells in normal human dermis. Br J Dermatol 1983 108: 423–431,  | PubMed | ChemPort |
  21. Hsu, SM, Raine, L, Tanger, M: A comparative study of the PAP method and avidin-biotin-complex method to study polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol 1981 75: 734–739,  | PubMed | ISI | ChemPort |
  22. Christensen, M, Briggs, RM, Coblentz, MG, Shapiro, MJ, Rikert, RR: Metastatic basal cell carcinoma: a review of the literature and report of two cases. Am Surg 1978 44: 382–387,  | PubMed | ChemPort |
  23. Safai, B, Good, RA: Basal cell carcinoma with metastasis. Arch Pathol Lab Med 1977 101: 327–331,  | PubMed | ChemPort |
  24. Sakula, A: Pulmonary metastasis from basal-cell carcinoma of skin. Thorax 1977 32: 637–642,  | PubMed | ChemPort |
  25. Murphy, GF, Ershler, WB, Krusinski, PA, Albertini, RJ: Evaluation of local and systemic immunocomponent cells in patients with multiple skin cancers (MSC). Clin Res 1982 30: 599A,
  26. Dvorak, AM, Mihm, MC Jr, Osage, BA, Dvorak, HF: Melanoma. An ultrastructural study of the host inflammatory and vascular responses. J Invest Dermatol 1980 75: 388–393,  | Article | PubMed | ChemPort |
  27. Hurrell, SM, Zarling, JM: Ly-2+ effectors cytotoxic for syngeneic tumor cells: generation by allogeneic stimulation and by supernatants from mixed leukocyte cultures. J Immunol 1983 131: 1017–1023,  | PubMed | ChemPort |
  28. Carter, RH, Drebin, JA, Schatten, S, Perry, LL, Greene, MI: Regulation of the immune response to tumor antigens. IX. In vitro Lyt-1+2- cell proliferative responses to cellbound or subcellular tumor antigen. J Immunol 1983 130: 997–1002,  | PubMed | ChemPort |

Extra navigation

.
ADVERTISEMENT