Original Article

Journal of Investigative Dermatology (1983) 80, 27–34; doi:10.1111/1523-1747.ep12530968

Basal Lamina Changes During Tissue Interactions in Hair Follicles—An In Vitro Study of Normal Dermal Papillae and Vitamin A-Induced Glandular Morphogenesis

Margaret H Hardy1, R J van Exan1,3, Karen S Sonstegard1,4 and P R Sweeny2

  1. 1Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada
  2. 2Department of Microbiology, University of Guelph, Guelph, Ontario, Canada
  3. 3Connaught Medical Research Laboartories, 1755 Steele Avenue West, Willowdale, Ontario, Canada
  4. 4National Institute of Environmental Health Sciences, Triangle Park, North Carolina

Received 9 June 1981; Accepted 26 April 1982.

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Abstract

Skin pieces from 14-day fetal mice were cultivated for 1-10 days prior to fixation and sectioning. Subsequently, sections were studied by light and transmission electron microscopy. In a standard medium the lateral hair follicle walls showed progressive maturation of the basal lamina, while the hair matrix, at the time of a known tissue interaction, showed the formation of gaps in the basal lamina, With heterotypic cell contacts through the gaps. In a vitamin-A enriched medium similar changes occurred, not only at the hair matrix, but also at lateral follicle walls, at the sites of, and prior to, budding and glandular morphogenesis. This study shows that the induction of hair matrix by dermal papilla may perhaps be added to the list of normal tissue interactions in which heterotypic cell contacts occur. It also suggests that vitamin-A induced glandular morphogenesis might come about through a mechanism resembling a normal tissue interaction.

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References

  1. Hardy, MH, Sweeny, PR, Bellows, CG: The effects of vitamin A on the epidermis of the fetal mouse in organ culture-an ultrastructural study. J Ultrastruct Res 1978 64: 246–260,  | Article | PubMed | ChemPort |
  2. Hardy, MH: Glandular metaplasia of hair follicles and other responses to vitamin A excess in cultures of rodent skin. J Embryol Exp Morphol 1968 19: 157–180,  | PubMed | ISI | ChemPort |
  3. Hardy, MH, Bellows, CG: The stability of vitamin A-induced metaplasia of mouse vibrissa follicles in vitro. J Invest Dermatol 1978 71: 236–241,  | Article | PubMed | ISI | ChemPort |
  4. Weiss, P: Principles of Development 1939 New York, Holt, Rinehart & Winston,
  5. Bellairs, R: Developmental Processes in Higher Vertebrates 1971 London, Logos Press,
  6. Kollar, EJ: The induction of hair follicles by embryonic dermal papillae. J Invest Dermatol 1970 55: 374–378,  | Article | PubMed | ISI | ChemPort |
  7. Dhouailly, D: Dermo-epidermal interactions between birds and mammals: differentiation of cutaneous appendages. J Embryol Exp Morphol 1973 30: 587–603,  | PubMed | ISI | ChemPort |
  8. Dhouailly, D: Formation of cutaneous appendages in dermo-epidermal recombinations between reptiles, birds and mammals. Roux's Archives 1975 177: 323–340,
  9. Hardy, MH: The differentiation of hair follicles and hairs in organ culture. Advances in Biology of Skin. Hair Growth 1969 9: Edited by W Montagna, RL Dobson. Oxford, Pergamon Press, pp 35–60,
  10. Wessells, NK: Tissue Interactions and Development 1977 Menlo Park, California, WA Benjamin,
  11. Hardy, MH, Sonstegard, KS, Sweeny, PR: Light and electron microscopic studies of the reprogramming of epidermis and vibrissa follicles by excess vitamin A in organ culture. In Vitro 1973 8: 405,
  12. Sporn, MB, Newton, DL: Chemoprevention of cancer with retinoids. Fed Proc 1979 38: 2528–2534,  | PubMed | ISI | ChemPort |
  13. Bollag, W: Retinoids and cancer. Cancer Chemother Pharmacol 1979 3: 207–215,  | PubMed | ChemPort |
  14. Grüneberg, H: The development of some external features in mouse embryos. J Hered 1943 34: 88–92,
  15. Hardy, MH: Responses in embryonic mouse skin to excess vitamin A in organotypic cultures from the trunk, upper lip and lower jaw. Exp Cell Res 1967 46: 367–384,  | Article | ChemPort |
  16. Wilkoff, LJ, Peckham, JC, Dulmadge, EA, Mowry, RW, Chopra, DD: Evaluation of vitamin A analogs in modulating epithelial differentiation of 13-day chick embryo metatarsal skin expiants. Cancer Res 1976 36: 964–972,  | PubMed | ChemPort |
  17. Sweeny, PR, Hardy, MH: Ciliated and secretory epidermis produced from embryonic mammalian skin in organ culture by vitamin A. Anat Rec 1976 185: 93–100,  | Article | PubMed | ChemPort |
  18. Van Exan, RJ: The development of the mammalian dermis: a light and electron microscope study in the foetal mouse. MSc Thesis 1976 University of Guelph,
  19. Van Exan, RJ: An in vitro study of the effects of excess vitamin A on the differentiation of the mammalian dermis. PhD Thesis 1979 University of Guelph,
  20. Davidson, P, Hardy, MH: The development of mouse vibrissae in vivo and in vitro. J Anat 1952 86: 342–356,  | PubMed | ISI | ChemPort |
  21. Kefalides, NA: Epilogue. Biology and Chemistry of Basement Membranes 1978 Edited by NA Kefalides. New York, Academic Press, pp 601–602,
  22. Slavkin, HC, Bringas, P: Epithelial-mesenchyme interactions during odontogenesis. IV. Morphological evidence for direct heterotypic cell-cell contacts. Dev Biol 1976 50: 428–442,  | Article | PubMed | ChemPort |
  23. Cutler, LS: Intercellular contacts at the epithelial-mesenchymal interface of the developing rat submandibular gland in vitro. J Embryol Exp Morphol 1977 39: 71–77,  | PubMed | ChemPort |

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