Original Article

Journal of Investigative Dermatology (1982) 79, 361–364; doi:10.1111/1523-1747.ep12529445

Autodegradation of 125I-Labeled Human Epidermal Cell Surface Proteins

Koji Hashimoto1, Kay Hiemstra Singer1 and Gerald S Lazarus2

  1. 1Division of Dermatology, Department of Medicine, Duke University Medical Center, Durham, North Carolina, U.S.A.
  2. 2Division of Immunology, Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina, U.S.A.

Received 28 September 1981; Accepted 5 April 1982.

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Abstract

Triton X-100 extracts of cultured human epidermal cells exhibited proteolytic activity as measured by the hydrolysis of [3H]-casein at neutral pH. The majority of endogenous proteolytic activity was inhibited by parahydroxy mercuribenzoate and by mersalyl acid, indicating the enzyme(s) was a thiol class proteinase(s). Crude Triton X-100 extracts were prepared from epidermal cell following labeling of proteins with 125I. Autodegradation of labeled proteins at 37° C was detected as early as 1 hr and reached a plateau level by 4 hr. Degradation was inhibited by thiol class proteinase inhibitors. Among the detergent-solubilized radiolabeled proteins a polypeptide chain of Mr 155,000 was particularly sensitive to degradation by endogenous thiol proteinase(s).

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