Original Article

Journal of Investigative Dermatology (1979) 73, 266–268; doi:10.1111/1523-1747.ep12531636

A Method for the Assay of Inflammatory Mediators in Follicular Casts

G F Webster1 and A M Kligman2

  1. 1Department of Pathology, School of Dental Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania U.S.A.
  2. 2Department of Dermatology, Duhring Laboratories, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania U.S.A.

Received 2 January 1979; Accepted 17 April 1979.

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Abstract

A method is presented whereby inflammatory mediators may be detected and quantified in individual follicular casts. Lysozyme, lactoferrin, IgG, IgM, C3 and material reacting with antiserum to polymorphonuclear leukocytes (PMN) were assayed by functional and immunologic methods. By these techniques, lysozyme, IgG and anti-PMN reactive material were detected in clinically uninflamed follicular casts from acne subjects.

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References

  1. Kligman, AM: An overview of acne. J Invest Dermatol 1974 62: 268–287,  | Article | PubMed | ISI | ChemPort |
  2. Lees, CW, Strauss, JS, Downing, DT, Pochi, PE, Bacta, M: Analysis of soluble proteins in comedones. Acta Dermatovenereol (Stockh) 1977 57: 117–120,  | ChemPort |
  3. Knop, J, Oleffs, K: Acne vulgaris: Anti-P acnes antibodies in comedones. Arch Dermatol Res 1978 261: 98,
  4. Hernandez, LG, Puhvel, SM: Analysis of the water soluble extract of comedones. J Invest Dermatol 1978 71: 330–333,  | Article | PubMed | ChemPort |
  5. Baggiolini, M, Hirsch, JG, DeDuve, C: Resolution of granules from rabbit heterophile leukocytes into distinct populations by zonal centrifugation. J Cell Biol 1969 40: 529–541,  | Article | PubMed | ChemPort |
  6. Marks, R, Dawber, RPR: Skin surface biopsy: An improved technique for the examination of the horny layer. Br J Dermatol 1971 84: 117,  | PubMed | ISI | ChemPort |
  7. Plewig, G, Kligman, AM: Acne, Morphogenesis and Treatment 1975 Springer-Verlag, NY, p 56,
  8. Holmes, HL, Williams, M, Cunliffe, WJ: Pilo-sebaceous duct obstruction and acne. Br J Dermatol 1972 87: 327–332,  | PubMed | ISI | ChemPort |
  9. Weissman, G, Zurier, H, Goldstein, IM: Mechanisms of lysosomal release from leukocytes exposed to immune complexes and other particles. J Exptl Med 1972 134: 149–165,
  10. Bennett, KM, Skosey, JL: Lactoferrin and lysozyme levels in synovial fluid differential indices of articular inflammation and degradation. Arthritis Rheum 1977 19: 84–90,
  11. Cimasoni, G, Ishikawa, I, Jaccura, F: Enzyme activity in the gingival crevice. The Borderland Between Carries and Periodontal Disease 1977 Edited by T. Lehner. Grune & Stratton, New York. p 24,
  12. Sugimato, M, Dannenberg, AM, Wahl, LM, Ettinger, WH, Hastie, At, Denials, DC, Demoulin-Brahy, C: Tuberculous lesions and tuberculin reactions collected in skin chambers. Am J Pathol 1978 90: 583–608,  | PubMed | ChemPort |
  13. Bennett, RM, Skosey, JL: Lactoferrin levels in synovial fluid: Its origin and significance as a marker of inflammation. J Rheumatol 1974 1: 44,
  14. Leffell, MS, Spitznagel, JK: Intercellular and extracellular degranulation of human polymorphonuclear azurophil and specific granules induced by immune complexes. Infect Immunol 1974 10: 1241–1249,  | ChemPort |
  15. Scheidegger, JJ: Une microméthode de l'immunoelectrophorèse. Int Arch All Appl Immunol 1955 7: 103–110,  | ChemPort |
  16. Osserman, ER, Lawlor, DP: Serum and urinary lysozyme (muramidase) in monocytic and monoelocytic leukemia. J Exptl Med 1966 124: 921–952,  | Article | ChemPort |
  17. Leffell, MS, Spitznagel, JK: Association of Lactoferrin with lysozyme in granules of human polymorphonuclear leukocytes. Infect Immunol 1972 6: 761–765,  | ChemPort |
  18. Puhvel, SM, Sakamoto, M: The chemoattractant properties of comedonal components. J Invest Dermatol 1978 71: 324–329,  | Article | PubMed | ChemPort |
  19. Webster, GF, Leyden, JJ, Norman, ME, Nilsson, UR: Complement activation in acne vulgaris: in vitro studies with Propionibacterium acnes and Propionibacterium granulosum and Propionibacterium granulosum. Infect Immunol 1978 22: 523–529,  | ChemPort |
  20. Webster, GF, Tsai, CC, Leyden, JJ: Neutrophil lysosomal release in response to Propionibacterium acnes. J Invest Dermatol 1979 72: 209,

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