Original Article

Journal of Investigative Dermatology (1974) 62, 510–513; doi:10.1111/1523-1747.ep12681038

MOUSE FOOT SCREEN FOR THE INHIBITION OF SWEATING BY ANTICHOLINERGIC DRUGS

Edwin Kaszynski and Susan Berney Frisch

Received 8 March 1972; Revised 1 November 1973; Accepted 5 November 1973.

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Abstract

Mice have been utilized to develop a simple, rapid, inexpensive, quantitative screening procedure for sweat inhibition by anticholinergic agents. Graded doses of anticholinergic drugs are injected with a microliter syringe subcutaneously under the plantar foot pads of one hind foot of mice. Both hind feet are then coated with a suspension of starch and castor oil, and the mice are stimulated to sweat by an intraperitoneal injection of 10 mg/kg of pilocarpine HCI. Sweating on the treated foot is judged to be inhibited or not inhibited relative to the contralateral untreated foot. The median inhibitory concentrations and 95% confidence limits are determined by a simple, approximate method. The relative potencies of test compounds are expressed in terms of their ratios to a standard, scopolamine HBr. Compounds representing a wide range of potencies have been screened, and significant differences in their sweat inhibiting potencies have been demonstrated. These compounds include, in order of decreasing activity: tridihexethyl chloride and (2-[2-methyl-1-pyrrol- idyl ]ethylphenylcyclopentylacetate) HCI, which have similar potencies, 3, 3-diphenyl-N- dimethylpropylamine HCI and hydroxyzine HCI.

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