Journal of Investigative Dermatology

TABLE 1

FROM:

Calcineurin Activity and Inhibition in Skin and (Epi)Dermal Cell Cultures

N P M Smit, H H Van Rossum, F P Romijn, K J Sellar, M Breetveld, S Gibbs and J Van Pelt

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Table 1. Activity and inhibition of Cn in cell cultures from two different donors

Cn activity 1 (nmol min- 1 mg- 1)
  Control + Trl  
Donor 1    % 2
 Fibroblasts12.73.326
 Keratinocytes7.71.924
    
Donor 2
 Keratinocytes8.51.011
 Melanocytes3.81.025

 Cn, calcineurin; Trl, Tacrolimus.

1 Fibroblasts and keratinocytes originating from one donor and keratinocytes and melanocytes originating from a second donor. Measurements were performed in duplicate cultures for donor 1 and single cultures for donor 2. Incubations were performed for 24 h with 124 nM Trl. Keratinocytes were grown in Fei medium.

2 The percentage (% ) activity compared to untreated control.

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