FIGURE 2

Histopathology of AD. Mice were killed at an age of 12 weeks and skin sections were evaluated with respect to epidermal hyperplasia (a and d, hematoxyllin/eosin staining), the presence of eosinophils (arrows) and neutrophils (b and e; LUNA staining), mast cells (c and f; toluidin blue staining), CD4+ T cells (g and j), CD11b⁺ cells (h and k), and IgE⁺ cells (i and l). (a–c and g–I) The results for APOC1(+/+) mice, whereas (d–f and j–l) show the staining of tissues from APOC1(+/-) controls. (m–o) The isotype controls for CD4, CD11b, and IgE, respectively. The insert of (a) shows an example of spongiosis at an age of 12 weeks. The arrow in (c) indicates degranulation of mast cells. Bar=50 m in (b, c, e, and f) and the insert of (a); bar=100 m in all other panels.