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Characterization of a Hapten-Induced, Murine Model with Multiple Features of Atopic Dermatitis: Structural, Immunologic, and Biochemical Changes following Single Versus Multiple Oxazolone Challenges
Mao-Qiang Man, Yutaka Hatano, Seung H Lee, Mona Man, Sandra Chang, Kenneth R Feingold, Donald Y M Leung, Walter Holleran, Yoshikazu Uchida and Peter M Elias
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Described Pathophysiological Features of Human AD and AD Model Mice
Supplementary Figure 1 (jpeg 110K)
Repeatedly Ox-Challenged Mice Develop a Pruritic Dermatosis: Gross appearance of hairless mice after one (1) vs. ten (10) Ox challenges
Supplementary Figure 2 (jpeg 262K)
Repeatedly Ox-Challenges Cause Epidermal Hyperplasia and Chronic Inflammation: A-C: H&E staining of vehicle-treated (A) and Ox-challenged skin sites after one (B) and 10 (C) challenges. D-F: PCNA staining of vehicle-treated (D) and Ox-challenged skin sites (E: one challenge; F: 10 challenges). Mag bars = 20 m.
Supplementary Figure 3 (jpeg 289K)
Alterations in Epidermal Differentiation after Multiple Ox Challenges: A-C: filaggrin; D-F: involucrin; G-I: loricrin, immunohistochemical staining, using DAB method, in 5 M paraffin sections. Mag bars = 20 m.
Supplementary Figure 4 (jpeg 105K)
Serine Protease (SP) Activity Increases and Extends into Nucleated Layers After Ten Ox Challenges. Frozen (5 ?m) sections were incubated with fluorescein-casein substrate at 37 C for 2 hrs. Brackets indicate zone of increased SP activity after ten Ox challenges (C).
Supplementary Figure 5 (jpeg 268K)
IL-4 Increase Levels in Repeatedly Ox-Challenged Mice. 5 m paraffin sections were incubated with monoclonal anti-mouse IL-4 antibody overnight at 40C, followed by incubation with secondary antibody, and ABC-peroxidase reaction (Vector, Burlingame, CA). Magnifications are indicated on the figure.