Journal of Investigative Dermatology

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Adrenocorticotropin Hormone Stimulates Interleukin-18 Expression in Human HaCaT Keratinocytes

Hyun Jeong Park, Hee Jung Kim, Jun Young Lee, Baik Kee Cho, Richard L Gallo and Dae Ho Cho

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Figure 1.

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Effects of ACTH on the mRNA expression and production of IL-18 in HaCaT keratinocytes. (a) HaCaT cells were harvested after ACTH treatment for 6 hours. Total RNA was extracted, and cDNA was synthesized for reverse transcriptase-PCR. The histogram shows the IL-18 mRNA expression level relative to beta-actin by densitometry. (b) Cells were treated with indicated concentration of ACTH for 24 hours. Cell supernatants were collected and then IL-18 concentration was measured by ELISA, each performed in triplicate. Data are meanplusminusSD. * P<0.05 versus control.

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