Journal of Investigative Dermatology

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UVA-Induced Modification of Catalase Charge Properties in the Epidermis Is Correlated with the Skin Phototype

Vittoria Maresca, Enrica Flori, Stefania Briganti, Emanuela Camera, Muriel Cario-André, Alain Taïeb and Mauro Picardo

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Figure 1.

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UVA is more effective than UVB in inducing modifications of enzymatic antioxidant activities in reconstructed epidermis and the phototype of melanocytes has a role in the photoprotection. Reconstructed epidermis was irradiated with UVA or UVB. The epidermis was homogenated and enzymatic activities were determined on the supernatants by spectrophotometrical techniques. REK=reconstructed epidermis with only keratinocytes; REML=reconstructed epidermis with melanocytes and keratinocytes from low phototype subjects; REMH=reconstructed epidermis with melanocytes and keratinocytes from high phototype subjects; CTR=not-irradiated samples; UVA=reconstructed epidermis irradiated with 8 J/cm2 UVA; UVB=reconstructed epidermis irradiated with 0.15 J/cm2 UVB. Each value is the meanplusminusSD of three determinations in duplicate. *P<0.05 in not-irradiated REML with respect to not-irradiated REMH; * P<0.05 in UV-treated reconstructed epidermis versus not-irradiated ones; ** P<0.005 in UV-treated reconstructed epidermis versus not-irradiated ones; *** P<0.001 in UV-treated reconstructed epidermis versus non-irradiated ones.

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