FIGURE 5
FROM:
Cornulin, a New Member of the "Fused Gene" Family, Is Expressed During Epidermal Differentiation
Romuald Contzler, Bertrand Favre, Marcel Huber and Daniel Hohl
BACK TO ARTICLEFigure 5.
(A) Constructs of expression vectors. (a) GFP-cornulin full length, (b) GFP-cornulin
EF, (c) GFP-EF-hand domain of cornulin, (d) HA-cornulin and (e) V5-Cornulin. Amino-terminal region containing EF-hand domains (squared box) and repeats (diagonal hatched boxes) are indicated as well as epitope site (horizontal black bar). (B) Cornulin is cytoplasmic and not a substrate for TG 1. HeLa cells were co-transfected with the indicated constructs and fixed. Cellular distribution was examined by GFP autofluorescence (left panel) and by staining with a mouse anti-TG 1 antibody (right panel). Cornulin has a cytoplasmic distribution and is not a substrate of TG 1, unlike control SPRR4 that colocalizes with this enzyme in the cell periphery. Control GFP exhibits a diffuse epifluorescence throughout the cytoplasm and nucleus.
