FIGURE 7
FROM:
Redox Imbalance Induced by Contact Sensitizers Triggers the Maturation of Dendritic Cells
Masato Mizuashi, Tomoyuki Ohtani, Satoshi Nakagawa and Setsuya Aiba
BACK TO ARTICLEFigure 7.
N-acetyl-L-cysteine (NAC) suppressed the phosphorylation of p38 mitogen-activated protein kinase (MAPK) in monocyte-derived dendritic cells (MoDC) stimulated with the contact sensitizers. MoDC were pretreated with 25 mM NAC for 30 min and then cultured with 300 
M or 1 mM of NiCl2 (a) or 10 or 30 M of 2,4-dinitrochlorobenzene (DNCB) (b). After 0–60 min of culture, the phosphorylation of p38 MAPK was analyzed by flow cytometry. The relative GSH/GSSG ratio was calculated as described in Materials and Methods. This is a representative flow cytometry of two independent experiments.
