FIGURE 6
FROM:
Redox Imbalance Induced by Contact Sensitizers Triggers the Maturation of Dendritic Cells
Masato Mizuashi, Tomoyuki Ohtani, Satoshi Nakagawa and Setsuya Aiba
BACK TO ARTICLEFigure 6.
N-acetyl-L-cysteine (NAC) increased the reduced/oxidized glutathione (GSH/GSSG) ratio in monocyte-derived dendritic cells (MoDC) with or without chemical stimulation. To examine the effects of the antioxidant NAC, we added 25 mM NAC to the culture of MoDC 30 min prior to stimulation by the chemicals. Two hours after culture, the relative GSH/GSSG ratio of MoDC was calculated as described in Materials and Methods. The mean
SEM of the relative GSH/GSSG ratio from three to seven independent experiments is shown. Asterisks indicate significance (**p<0.01, *p<0.05) for the difference between MoDC treated with NAC and those untreated with NAC.
