Journal of Investigative Dermatology

FIGURE 2

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Interleukin-6 Induced Basic Fibroblast Growth Factor-Dependent Angiogenesis in Basal Cell Carcinoma Cell Line via JAK/STAT3 and PI3-Kinase/Akt Pathways

Shiou-Hwa Jee, Chia-Yu Chu, Hien-Ching Chiu, Yi-Ling Huang, Wei-Ling Tsai, Yi-Hua Liao and Min-Liang Kuo

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Figure 2.

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Upregulation of basic fibroblast growth factor 2 (bFGF) in basal cell carcinoma (BCC) cells over-expressing interleukin-6 (IL-6). (A) After serum starvation, the mRNA of various cell lines as indicated were extracted and semiquantified by RT-PCR of bFGF, platelet-derived growth factor (PDGF), and IL-8. The number below each lane indicates the relative densitometric intensity to control (defined as 1.0). This represents one of three experiments. (B). Cell-free culture supernatant was analyzed for bFGF by ELISA. Concentration of bFGF was expressed as meanplusminusSD of five experiments. Student's t test was used for comparison.

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