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Expression of CD200 on Epithelial Cells of the Murine Hair Follicle: A Role in Tissue-Specific Immune Tolerance?
Michael D Rosenblum, Edit B Olasz, Kim B Yancey, Jeffrey E Woodliff, Zelmira Lazarova, Kimberly A Gerber and Robert L Truitt
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Actuarial graft-survival curves, CD200-11-(squares; n=20) and wild-type (WT) (triangles; n=19) B6 skin was grafted onto individual WT recipients. Grafts were censored from the analysis on the day that they were biopsied to avoid skewing the outcome since the process appeared to trigger rejection in a few CD200-/- grafts but not in any of the WT grafts.
Figure S2 (jpeg 23K)
Gating strategy used for flow cytometric analysis of CD4 and CD8 T cells in wild-type (WT) and CD200-deficient skin grafts. Debris and dead cells were excluded on the basis of forward light scatter (FSC) and uptake of 7AAD ("R1" in upper left panel). An electronic gate ("R2") was set around lymphocyte and lymphoblastic populations within R1 based on FSC and side scatter (SSC) (upper right panel). Cells falling within both R1 and R2 were then analyzed for expression of CD4 and CD8 (as shown in text Figure 5). Unstained cells (negative controls) from WT and CD200-/- mice are shown in the bottom panels.
Figure S3 (jpeg 14K)
Isotype control staining for immunohistochemistry of wild-type C57BL/6 neonatal pup skin (right panel) as compared to the positive control stained with CD200-specific antibody (left panel). Immunohistochemistry was performed on frozen sections as described in Materials and Methods.
