FIGURE 3

Primary sequence and expression of rWise. (A) Primary sequence of rat Wise, the black underline is the secretion motif, the black box is the cysteine knot region, and the gray box is the region to which the AK-1 antibody was raised. (B) Localization of Wise transcripts in the anagen vibrissa. O, outer root sheath; i, inner root sheath; dp, dermal papilla; Hs, hair shaft. (C) Sense control, m, melanin pigment. (D) Detail of dermal papilla cells from anti-sense (AS) and sense (S) hybridizations. (E) Localization of Wise transcripts in the anagen pelage follicle. ors, outer root sheath; ds, dermal sheath. In all cases, the dermal papilla is specified by dotted lines. (F) Expression of Myc-Tagged rWise in 3T3 cells. (G) Western blots of Triton X-100 soluble (s) and insoluble (i) fractions of lysates from WISE- and vector-transfected 3T3 cells incubated with either anti-myc or the AK-1 antibody. IVT, in vitro translation product of rWise RNA. Scale bars, 100 m (B–D) and 10 m (E, F).