FIGURE 5
FROM:
A Synthetic Superoxide Dismutase/Catalase Mimetic (EUK-134) Inhibits Membrane-Damage-Induced Activation of Mitogen-Activated Protein Kinase Pathways and Reduces p53 Accumulation in Ultraviolet B-Exposed Primary Human Keratinocytes
David Decraene, Katrien Smaers, David Gan, Tom Mammone, Mary Matsui, Daniel Maes, Lieve Declercq and Marjan Garmyn
BACK TO ARTICLEFigure 5.
Inhibition of the p38 MAPK pathway reduces p53 N-terminal phosphorylation and p53 accumulation following UVB irradiation. Half an hour before UVB irradiation, cells were pre-treated with increasing concentrations of the p38 MAPK inhibitor PD169316 (1–10 
M). Cells were then irradiated with 32 mJ per cm2 UVB and scraped at the indicated time point. p53 phosphorylation at serine 46, p53 phosphorylation at serine 15, and total p53 level was determined by western blotting as described under Materials and Methods.
