FIGURE 4
FROM:
Interleukin-3R
+ Myeloid Dendritic Cells and Mast Cells Develop Simultaneously from Different Bone Marrow Precursors in Cultures with Interleukin-3
Thomas Baumeister, Susanne Rö
ner, Gabi Pech, MarellaFTR de Bruijn, Pieter J M Leenen, Gerold Schuler and Manfred B Lutz
Figure 4.
IL-3/DC and GM-CSF/DC produce similar amounts of IL-12. (a), (b) BM cells were cultured with IL-3 or GM-CSF until day 8 and then incubated overnight with cytoperm solution and LPS for cytokine production. Then, the cells were fixed and stained intracellularly for IL-12 p40 (continuous line) or an isotype control monoclonal antibody (dotted line) and on the surface for CD11c. (a) Histograms for IL-12 p40 staining of gated CD11c+ and CD11c– cells. Both subsets produce IL-12 p40. (b) GM-CSF/DC were double stained for MHC II, indicating that only the most mature DC (MHC IIhigh) produce IL-12 p40. (c) IL-3/DC and GM-CSF/DC were cultured until day 8 and then stimulated with SAC alone or in combination with LPS and IFN-
to detect IL-12 p70 after 48 h by ELISA.
