FIGURE 2

DHLA, LA, and resveratrol inhibit forskolin and UVB-induced MITF promoter activity. B16 cells transfected with the MITF promoter-luciferase construct (pGL2.hMIP) were treated 48 h after transfection, with (a) Increasing concentrations of DHLA, LA, and resveratrol. (b) Forskolin alone (100 M), or in combination with DHLA (300 and 400 M), LA (400 M), or resveratrol (20 and 30 g per ml); (c) UVB alone (30 mJ per cm²), or in combination with DHLA (200 and 400 M). Measured luciferase activity is expressed as percent increase of MITF promoter activity relative to untreated control (meanSEM). (d) B16 cells were treated with TCM-1, DHLA, forskolin, and cell permeable 8-bromo-cAMP at the concentrations indicated for 7 h. Measured cellular cAMP is expressed as percent increase of cAMP concentration relative to untreated control (meanSEM).