1. Basic Research Laboratory, Kanebo Ltd., Kanagawa, Japan
2. ^*Department of Dermatology, Gunma University School of Medicine, Gunma, Japan

Correspondence: Dr Shintaro Inoue, Basic Research Laboratory, Kanebo Ltd., 5–3-28 Kotobuki-cho, Odawara, Kanagawa 250–0002, Japan. Email: inoshin@oda.cos.kanebo.co.jp

Received 27 January 2000; Revised 16 March 2000; Accepted 18 March 2000.

Abstract

Hyaluronan is well known to exist as a water-sorbed macromolecule in the extracellular matrix. We here examined whether hyaluronan exists in the normal stratum corneum. High performance liquid chromatography was used to quantify hyaluronan content in the stratum corneum, epidermis (including stratum corneum), and dermis of mice, with the resulting dry weights being 22.3  2.9, 15.1  1.5, and 738.6  31.6 g per g, respectively. Normal mouse skin was then labeled with [³H]-glucosamine in an organ culture, and accumulation of [³H]-labeled hyaluronan and its molecular mass were determined separately for the stratum corneum, epidermis, and dermis. In the stratum corneum, [³H]-labeled hyaluronan was accumulated linearly over the 3-d culture period. After the 3-d culture period, the epidermis synthesized twice the amount (expressed as dpm per mg dry weight) of [³H]-labeled hyaluronan as the dermis, whereas the stratum corneum and dermis showed nearly the same content of [³H]-labeled hyaluronan. The molecular mass of [³H]-labeled hyaluronan was highest (>1.0  10⁶) in the dermis and clearly lower (<6.0  10⁴) in the stratum corneum. Based on these results, we here confirm that hyaluronan is supplied from keratinocytes beneath the stratum corneum layer, and is present in the normal stratum corneum. We speculate that hyaluronan may play a role in moisturizing the stratum corneum and/or regulating its mechanical properties.