Original Article

Journal of Investigative Dermatology (1996) 106, 129–134; doi:10.1111/1523-1747.ep12329620

Multiparametric Image Cytometry in Mycosis Fungoides

Matthew G Fleming1 and Thomas W Rauber2

  1. 1Department of Dermatology, Medical College of Wisconsin and Veterans Administration Medical Center, Milwaukee, Wisconsin, U.S.A.
  2. 2New University of Lisbon, Faculty of Sciences and Technology and UNINOVA—Intelligent Robotics Center, Quinta da Torre, Monte de Caparica, Portugal

Received 6 May 1995; Revised 9 September 1995; Accepted 15 September 1995.

Top

Abstract

Eighteen cases of early mycosis fungoides were compared with 18 cases of eczematous dermatitis by multiparametric image cytometry. A minimum of 100 lymphocytes was measured in each case. A large number of measurements was acquired for each lymphocyte, characterizing nuclear DNA content, area, shape, and texture. There were significant differences between the two groups, especially in nuclear DNA content and texture. These differences allowed the two groups of nuclei to be distinguished with 78% accuracy. The two groups of lesions were distinguished with 94% accuracy, using neural network analysis.

Keywords:

chromatin texture, lymphoma, image analysis, DNA-neoplasm

Top

References

  1. Wood, GS, Haeffner, A, Dummer, R, Crooks, CF: Molecular biology techniques for the diagnosis of cutaneous T-cell lymphoma. Dermatol Clin 12: 231–241, 1994
  2. Weiss, LM, Hu, E, Wood, GS, Moulds, C, Cleary, ML, Warnke, R, Sklar, J: Clonal rearrangements of T cell receptor genes in mycosis fungoides and dermatopathic lymphadenopathy. N Engl J Med 313: 537–544, 1985
  3. Zelickson, BS, Peters, MS, Muller, SA, Thibodeau, SN, Lust, JA, Quam, LM, Pittelkow, MR: T-cell receptor gene rearrangement analysis: cutaneous T cell lymphoma, peripheral T cell lymphoma, and premalignant and benign cutaneous lymphoproliferative disorders. J Am Acad Dermatol 25: 787–796, 1991 | PubMed | ISI | ChemPort |
  4. Dosaka, N, Tanaka, T, Fujita, M, Miyachi, Y, Horio, T, Imamura, S: Southern blot analysis of cloual rearrangements of T-cell receptor gene in plaque lesion of mycosis fungoides. J Invest Dermatol 93: 626–629, 1989
  5. Wood, GS, Tung, RM, Haeffner, AC, Crooks, CF, Liao, S, Orozco, R, Veelken, H, Kadin, ME, Koh, H, Heald, P, Harnhill, RL, Sklar, J: Detection of T-cell receptor gamma gene rearrangements in early mycosis fundoides/Sezary syndrome by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/ DGGE). J Invest Dermatol 103: 34–41, 1994 | Article | PubMed | ISI | ChemPort |
  6. McNutt, NS, Heilbron, DC, Crain, WR: Mycosis fungoides. Diagnostic criteria based on quantitative electron microscopy. Lab Invest 44: 466–474, 1981
  7. van der Loo, EM, van Vloten, WA, Cornelisse, WA, Scheffer, E, Meijer, CJLM: The relevance of morphometry in the differential diagnosis of cutaneous T cell lymphomas. Br J Dermaatol 104: 257–269, 1981
  8. Hantanyapong, Y, Iwahara, K, Zhuan, KG, Yaguchi, H, Nakijima, S, Ogawa, H, Seou, Y, Takiuchi, I: Comparison of the nuclear contour index (NCI) and T cell subsets of erythematosus, plaque and tumor lesions in patients with mycosis fungoides. J Dermatol 14: 43–47, 1987
  9. Shum, DT, Roberts, JT, Smout, MS, Wells, GA, Simon, GT: The value of nuclear contour index in the diagnosis of mycosis fungoides. An assessment of current ultrastructural diagnostic criteria. Cancer 57: 298–304, 1986
  10. van Vloten, WA, Schaberg, A, van der Ploeg, M: Cytophotometric studies on mycosis fungoides and other cutaneous reticuloses. Bull Cancer 64: 249–258, 1977
  11. Hagedorn, M, Kiefer, G: DNA content of mycosis fungoides cells. Arch Derm Res 258: 127–134, 1977
  12. Ralfkiaer, E, Larsen, JK, Christensen, IJ, Thomsen, K, Wantzin, GL: DNA analysis by flow cytometry in cutaneous T-cell lymphomas. Br J Dermatol 120: 597–605, 1989
  13. Pastel-Levy, C, Flotte, TJ, Preffer, F, Ware, A, Graeme-Cook, F, Bell, DA, Colvin, RB: Application of DNA flow cytometry from paraffin-embedded tissue to the diagnosis of mycosis fungoides. J Cutan Pathol 18: 279–283, 1991
  14. Brugal, G, Quirion, C, Vassilakos, P: Detection of bladder cancers using a SAMBA 2000 cell image processor. Anal Quant Cytol Histol 8: 187–194, 1986
  15. Galera-Davidson, H, Bibbo, M, Bartels, PH, Dytch, HE, Gonzalez-Campora, R, Sanchez, F, Wied, GL: Differential diagnosis between follicular adenoma and follicular carcinoma of the thyroid by marker features. Anal Quant Cytol Histol 8: 195–200, 1986
  16. Salmon, I, Kiss, R, Franc, B, Gasperin, P, Heimann, R, Pasteels, JL, Verhest, A: Comparison of morphonuclear features in normal, benign and neoplastic thyroid tissue by digital cell image analysis. Anal Quant Cytol Histol 14: 47–51, 1992
  17. Verhest, A, Kiss, R, D'Olne, D, Larsimont, D, Salmon, I, Launoit, Y, Fourneau, C, Pasteels, JL, Pector, JC: Characterization of human colorectal mucosa, polyps, and cancers by mean of computerized morphonuclear image analyses. Cancer 65: 2047–2054, 1990
  18. Rickaert, F, Gelin, M, van Gansbeke, D, Lambilliotte, JP, Verlhest, A, Pasteels, JL, Klooppel, G, Kiss, R: Computerized morphonuclear characteristics and DNA content of adenocarcinoma of the pancreas, chronic pancreatitis, and normal tissues: relationship with histopathologic grading. Hum Pathol 23: 1210–1215, 1992
  19. Christen, R, Xiao, J, Minimo, C, Gibbons, G, Fitzpatrick, BT, Galera-Davidson, H, Bartels, PH, Bibbo, M: Chromatin texture features in hematoxylin and cosinstained prostate tissue. Anal Quant Cytol Histol 15: 383–388, 1993
  20. Fleming, MG, Friedman, RJ: Multiparametric image cytometry of nevi and melanomas. Am J Dermatopathol 15: 106–113, 1993
  21. Gunzer, U, Harms, H, Haucke, M, Aus, HM, ter Meulen, V: Computer-aided image analysis for the differentiation of mononuclear cells in peripheral blood smears from leukemic patients. Anal Quant Cytol Histol 3: 26–32, 1981
  22. Durie, BGM, Vaught, L, Chen, YP, Olson, GB, Salmon, SE, Bartels, PH: Discrimination between human B and T lymphocytes and monocytes by computer analysis of digitized data from scanning microphotometry. I. Chromatin distribution patterns, Blood 51: 579–589, 1978
  23. Sahmoud, TM, Heudes, D, Irinopoulou, T, Gluckman, E, Nguyen, Q, Brocheriou, C, Devergie, A, Rigaut, JP, Mary, JY: Towards an Objective prognostic index of acute graft-versus-host disease. Anal Cell Pathol 5: 289–297, 1993
  24. Wiseman, CB: Cytotechnology Tech Sample No. CY-t 1–6. American Society of Clinical Pathologists, Chicago, 1986
  25. Fleming, MG: Design of a high resolution image cytometer with open software architecture. Anal Cell pathol (In Press),
  26. Jordon, R: The Khoros Group: Khoros. A software development environment for data processing. DSP Applications 2: 16–23, 1993
  27. Konstantinides, K: The Khoros software development environment for image and signal processing. IEEE Tram Image Proc 3: 243–252, 1994
  28. Rasure, J, Argiro, D, Sauer, T, Williams, C: Visual language and software development environment for image processing. Int J Imaging Systems and Technology 2: 183–199, 1990
  29. Haralick, RM, Shanmugam, K, Dinstein, I: Textural features for image classification. IEEE Trans Syst Man Cybern SMC 3: 610–621, 1973
  30. Gonzalez, RC, Wood, RE: Digital Image Processing 1993 Addison-Wesley, Reading, MA, pp 514–518
  31. Delfino, VP, Lettini, T, Vacca, E, Potente, F, Ragone, P, Ricco, K: Biological image analysis for microscopical diagnosis: The workstation S.A.M. (Shape Analytical Morphometry). Path Res Pract 188: 587–592, 1992
  32. Nafe, R, Kaloutsi, V, Choritz, H, Georgii, A: Elliptic Fourier analysis of megakaryocyte nuclei in chronic myeloproliferative disorders. Anal Quant Cytol Histol 14: 391–397, 1992
  33. Resta, L, Ricco, K, Colucci, GA, Troia, M, Russa, S, Vacca, E, Garofalo, G, Delfino, V: Multiparametric discrimination of serous ovarian tumors by analytical morphometry. Eur J Gynaecol Oncol 13: 60–64, 1992
  34. Nafe, R, Roth, S, Rather, P: Fourier analysis as a phanimetric procedure-application to malignant and normal urothelial cells with reactive changes. Exp Pathol 43: 155–161, 1991
  35. Ricco, R, Serio, G, Caniglia, GM, Cimmino, A, Lettini, T, Lozupone, A, Delfino, VP: Size and shape evaluation of astrocytoma nuclei with the shape analytical morphometry system. Anal Quant Cytol Histol 16: 345–350, 1994
  36. Messerla, ME: 4K 24-bit color capture for arts & sciences: the difference it makes. Advanced Imaging 8: 45–71, 1993
  37. Garner, DM, Harrison, A, MacAulay, C, Palcie, B: Cyto-SavanttradeTM and its use in automated screening of cervical smears. Anal Quant Cytol Histol. Compendium on the Computerized Cytology and Histology Laboratory 346–352, 1994
  38. Bagot, M, Wechsler, J, Leses, MC, Revuz, J, Parcet, JP, Gaulard, P: Intraepidermal localization of the clone in cutaneous T-cell lymphoma. J Am Acad Dermatol 27: 589–593, 1992 | PubMed | ISI | ChemPort |
  39. Fivenson, DP, Hanson, CA, Niekoloff, BJ: Localization of the clonal T cells to the epidermis in cutaneous T-cell lymphoma. J Am Acad Dermatol 31: 717–723, 1994 | PubMed | ISI | ChemPort |
  40. Klawe, H, Rowinski, J: Malignancy associated changes (MAC) in cells of buccal smears detected by means of objective image analysis. Acta Cytologica 18: 30–33, 1974
  41. Bibbo, M, Bartels, PH, Sychra, JJ, Wied, GL: Chromatin appearance in intermediate cells from patients with uterine cancer. Acta Cytologica 25: 23–28, 1981
  42. Burger, G, Jutting, U, Kodenacker, K: Changes in benign cell populations in cases of cervical cancer and its precursors. Anal Quant Cytol Histol 3: 261–271, 1981
  43. Bibbo, M, Montag, AG, Lerma-Puertas, E, Dytch, HE, Leelakusolvong, S, Bartels, PH: Karyometric marker features in tissue adjacent to invasive cervical carcinomas. Anal Quant Cytol Histol 11: 281–285, 1989 | PubMed | ISI | ChemPort |
  44. Bibbo, M, Bartels, PH, Galera-Davidson, H, Dytch, HE, Wied, GL: Markers for malignancy in the nuclear texture of histologically normal tissue from patients with Thyroid tumors. Anal Quant Cytol Histol 8: 168–176, 1986
  45. Galera-Davison, H, Bartels, PH, Fernandez-Rodriguoz, A, Dytch, HE, LermaPuertas, E, Biboo, M: Karyometric marker features in fine needle aspirates of invasive fullicular carcinoma of the thyroid. Anal Qnant Cytol Histol 12: 35–41, 1990
  46. Montag, AG, Bartels, PH, Dytch, HE, Lenna-Puertas, E, Michelassi, F, Bibbo, M: Karyometric features in nuclei near colonic adenocarcinoma. Statistic analysis. Anal Quant Cytol Histol 13: 159–167, 1991
  47. Wied, GL, Bartels, PH, Bibbo, M, Dytch, HE: Image Analysis and Quantitative Cytoand Histopathology 1988, International Academy of Cytology, Chicago, technical report no. 2
  48. Klecka, WR: Discriminant Analysis Sage Publications. Beverly Hills, CA, 1976
  49. Stolz, W, Abmayr, W, Schmoeckel, C, Landthaler, M, Massoudy, P, Braun-Falco, O: Ultrastructural discrimination between malignant melanomas and benign nevocytic nevi using high-resolution image and multivariate analysis. J Invest Dermatol 97: 903–910, 1991

Extra navigation

.
ADVERTISEMENT