Original Article

Journal of Investigative Dermatology (1995) 104, 199–203; doi:10.1111/1523-1747.ep12612755

Co-Administration of Insulin-Like Growth Factor (IGF)-I and IGF-Binding Protein-1 Stimulates Wound Healing in Animal Models

Ryoji Tsuboi1, Chog-Ming Shi1, Chiyo Sato1, George N Cox2 and Hideoki Ogawa1

  1. 1Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan
  2. 2Synergen, Inc., Boulder, Colorado, U.S.A.

Received 20 July 1994; Revised 15 October 1994; Accepted 11 November 1994.

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Abstract

The stimulatory effect of recombinant human insulin-like growth factor-I (rhIGF-I) and recombinant human insulin-like growth-factor-binding protein-1 (rhIGFBP-1) on wound healing was assessed using diabetic db/db mice and normal rabbits. Full-thickness wounds of 6 mm diameter were prepared on the backs of diabetic C57BL/KsJ db/db mice and on the inner sides of normal rabbit ears. Various concentrations of rhIGF-I and/or rhIGFBP-1 were applied locally to the open wounds of db/db mice once daily for 5 d and to the covered wounds of normal rabbits once after wounding. Sections of the wounds were evaluated histologically on the seventh or eighth day by measuring re-epithelialization (%), area of granulation tissue (mm2), and capillary numbers. Wound repair was accelerated by each of the treatments in descending order of rhIGF-I plus rhIGFBP-1, rhIGF-I, rhIGFBP-1, and vehicle alone. In db/db mice, the combination of 50 mug rhIGF-I and 165 mug rhIGFBP-1 (equimolar ratio) significantly stimulated granulation tissue formation (p < 0.01) and capillary numbers (p < 0.05). Doses of rhIGFBP-1 greater than 16.5 mug were required for significant acceleration of the healing stimulated by 50 mug of rhIGF-I. In normal rabbits, co-administration of 10 mug rhIGF-I and 33 mug rhIGFBP-1 (equimolar ratio) significantly stimulated all three wound-healing parameters (p < 0.01), with such stimulation being much greater than that induced by rhIGF-I alone. Interestingly, rhIGFBP-1 alone showed a mild stimulatory activity on wound healing in both models despite its lack of mitogenic activity in vitro. These results demonstrate that rhIGFBP-1 enhances the stimulatory activity of rhIGF-I on wound healing and suggest the clinical utility of the co-administration of rhIGF-I and rhIGFBP-1 for wound repair.

Keywords:

diabetic mouse, rabbit ear, IGFBP-1

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