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Journal of Investigative Dermatology (1994) 102, 833–837; doi:10.1111/1523-1747.ep12382086

Characterization of Expression and Modulation of Cell Adhesion Molecules on an Immortalized Human Dermal Microvascular Endothelial Cell Line (HMEC-1)

Yuelin Xu1, Robert A Swerlick1, Norbert Sepp1, Diane Bosse2, Edwin W Ades2 and Thomas J Lawley1

  1. 1Department of Dermatology, Emory University School of Medicine, Atlanta, Georgia, U.S.A.
  2. 2Biological Products Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, U.S.A.

Received 14 October 1993; Accepted 24 January 1994.

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Abstract

We have recently reported the creation of the first immortalized cell line derived from human dermal microvascular endothelial cells (HMEC-1). In preliminary studies this line was found to closely resemble microvascular endothelial cells in regard to many phenotypic characteristics. Because two key functional features of endothelial cells are their ability to bind to peripheral blood leukocytes and extracellular matrix proteins via cell adhesion molecules, we have now characterized HMEC-1 in terms of expression and regulation of cell adhesion molecules of the integrin, immunoglobulin gene superfamily, and selectin families. HMEC-1 can either constitutively express or can be induced to express key integrins, including alpha-1, -2, -3, -4, -5, -6, and -V, as well as beta-1, -3, -4, and -5. They also express or are capable of expressing immunoglobulin gene superfamily molecules, such as intercellular adhesion molecule-1 and vascular cell adhesion molecule-1, and a member of the selectin family, E-selectin. A number of important cell adhesion molecules that are either constitutively expressed or that must be induced are regulated in a time- and dose-dependent fashion by selected cytokines. Experiments comparing the phenotypic characteristics of HMEC-1 with human dermal microvascular endothelial cells or human umbilical vein endothelial cells reveal HMEC- 1 to have features of both small- and large-vessel endothelial cells.

Keywords:

cell adhesion molecules, integrins, human dermal microvascular endothelial cell line

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