Original Article

Journal of Investigative Dermatology (1993) 101, 138S–142S; doi:10.1111/1523-1747.ep12363264

Effect of Retinoids on Follicular Cells

Gail Bazzano1,2, Nia Terezakis1,2, Hala Attia1,2, Alicia Bazzano1,2, Robin Dover3, David Fenton3, Nikki Mandir3, Leonardo Celleno4, Maria Tamburro4 and Stefano Jaconi5

  1. 1Department of Dermatology, Tulane University Medical School, New Orleans, Louisiana, U.S.A.
  2. 2Touro Infirmary Research Department, New Orleans, Louisiana, U.S.A.
  3. 3Imperial Cancer Research Fund, Histopathology Unit, London, England
  4. 4Universita Cattolica del Sacro Cuore Department of Dermatology, Rome, Italy
  5. 5Clinique de Dermatologie, Hôpital Cantonal Universitaire, Geneva, Switzerland
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Abstract

It has been demonstrated that topical application of all-trans retinoic acid and other retinoids can alter the hair-growth cycle in the C3H mouse model. The anagen phase is prolonged and the telogen phase is shortened. This effect is similar to the effect of minoxidil on the hair-cycle dynamics in this animal model.

The levels of cellular retinoic acid binding protein measured by radioreceptor assay in whole skin of C3H mice were higher during anagen and lower during telogen. Topical application of certain retinoids caused elevated levels of cellular retinoic acid-binding protein (cRABP) in the whole skin homogenates during both phases of the cycle. Of the retinoids tested, those most effective in altering the levels of cRABP in the skin of the mice were also capable of significantly altering the hair-cycle dynamics. There appeared to be a relationship between the ability of retinoid to increase cRABP, increase 3H-thymidine incorporation, and alter the dynamics of the hair cycle.

Only cRABP-II is detectable in human cultured dermal fibroblasts and dermal papilla cells. Dermal fibroblasts showed higher amounts of cRABP-II as compared to dermal papilla cells. The difference in cRABP-II expression might explain a distinct response to RA by these two cell populations. Whether the difference in expression of cRABP-II might be of physiologic importance remains to be determined.

Treatment of human dermal papilla cells in culture with retinoic acid does not appear to affect proliferation, at least at the doses tested.

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References

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