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Effects of lead exposure on the status of platelet indices in workers involved in a lead-acid battery manufacturing plant

Journal of Exposure Science & Environmental Epidemiology volume 24, pages 629–633 (2014)Cite this article

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Abstract

This study was carried out to determine the effect of Pb exposure on the status of platelet indices in workers exposed to Pb during lead-acid battery plant process. Platelet indices and blood lead levels (BLLs) were determined in 429 male workers. BLLs were determined by using an atomic absorption spectrophotometer. Platelet indices in the samples were quantified by using the Sysmex KX-21 hematology analyzer. The levels of platelet count (PLT), plateletcrit (PCT) and mean platelet mass (MPM) were significantly decreased and platelet distribution width (PDW), platelet large cell ratio (P-LCR) and mean platelet volume were increased with an increase in BLLs. The results of linear multiple regression analysis showed that the platelet count (β −0.143, P=0.005), PCT (β −0.115, P=0.023) and MPM (β −0.110, P=0.030) were negatively associated with BLLs and P-LCR (β 0.122, P=0.016) was positively associated with BLLs. The variable of body mass index showed a positive association with PCT (β 0.105, P=0.032) and MPM (β 0.101, P=0.039). The results of the study may indicate that lead exposure may impair coagulation function through endothelial tissue injury and reduction of nitric oxide.

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References

  1. Okediran BS, Abam E, Odukoya OO, Adamson I, Ademuyiwa O . Membrane intracellular, plasma and urinary sodium and potassium in occupational lead exposure: effects of vitamin C supplementation. Trace Element Electrolytes 2009; 26: 49–59.

    Article  CAS  Google Scholar 

  2. Tuakuila Joel, Lison Dominique, Mbuyi Francois, Haufroid Vincent, Hoet Perrine . Elevated blood lead levels and sources of exposure in the population of Kinshasa, the capital of democratic republic of Congo. J Exposure Sci Environ Epidemiol 2013; 23: 81–87.

    Article  CAS  Google Scholar 

  3. Tong S, von Schirnding YE, Prapamontol T . Environmental lead exposure: a public health problem of global dimensions. Bull World Health Organization 2000; 78: 1068–1077.

    CAS  Google Scholar 

  4. Agency for Toxic Substances and Diseases Registry (ATSDR). Toxicological Profile for Lead. U.S. Department of Health and Human Services. Public Health Service: Atlanta, GA. 2010.

  5. Paul Harrison . Review-Platelet function analysis. Blood Rev 2005; 19: 111–123.

    Article  Google Scholar 

  6. Chu SG, Becker RC, Berger PB, Bhatt DL, Eikelboom JW, Konkle B et al. Mean platelet volume as a predictor of cardiovascular risk: a systematic review and meta analysis. J Thromb Haemost 2010; 8: 148–156.

    Article  CAS  Google Scholar 

  7. Rojas-Garcia AE, Medina-Diaz IM, Robledo-Marenco Mde L, Barron-Vivanco BS, Giron-Perez MI, Velazquez-Fernandez JB et al. Hematological, biochemical effects, and self-reported symptoms in pesticide retailers. J Occup Environ Med 2011; 53: 517–521.

    Article  CAS  Google Scholar 

  8. Azmi MA, Naqvi SN, Akhtar K, Moinuddin, Parveen S, Parveen R et al. Effect of pesticide residues on health and blood parameters of farm workers from rural Gadap, Karachi, Pakistan. J Environ Biol 2009; 30: 747–756.

    CAS  PubMed  Google Scholar 

  9. Smith D.L, Petruzzello S.J, Goldstein E, Ahmad U, Tangella K, Freund GG et al. Effect of live-fire training drills on firefighters' platelet number and function. Prehosp Emerg Care 2011; 15: 233–239.

    Article  Google Scholar 

  10. Mojiminiyi FB, Merenu IA, Ibrahim MT, Njoku CH . The effect of cement dust exposure on hematological and liver function parameters of cement factory workers in Sokoto, Nigeria. Niger J Physiol Sci 2008; 23: 111–114.

    CAS  PubMed  Google Scholar 

  11. Chen JC, Chen YJ, Chang WP, Christiani DC . Long driving time is associated with hematological markers of increased cardiovascular risk in taxi drivers. Occup Environ Med 2005; 62: 890–894.

    Article  Google Scholar 

  12. Nowakowska B, Prazanowski M, Palmowska M, Szymczak W . The effect of occupational exposure to metallic mercury on selected parameters of hemostasis. Med Pr 1997; 48: 529–538.

    CAS  PubMed  Google Scholar 

  13. Kavita Marita G, Kandregula Sandeep, Lakshmana Rao N . Acute noise exposure effect on certain hematological parameters in tailors: a pilot study. Int J Biol Med Res 2012; 3: 1529–1531.

    Google Scholar 

  14. Krishnan RM, Sullivan Jeffrey H, Carlsten Chris, Wilkerson Hui-Wen, Beyer Richard P, Bammler Theo et al. A randomized cross-over study of inhalation of diesel exhaust, hematological indices, and endothelial markers in humans. Particle Fibre Toxicol 2013; 10: 1–10.

    Article  Google Scholar 

  15. Naza Mohammad Ali Mahmood. Relationship between exposure to petrol products and the trace metal status, liver toxicity and hematological markers in gasoline filling workers in Sulaimani city. J Environ Occup Sci 2012; 1: 6–11.

    Article  Google Scholar 

  16. Uzma N, Salar BM, Kumar BS, Aziz N, David MA, Reddy VD . Impact of organic solvents and environmental pollutants on the physiological function in petrol filling workers. Int J Environ Res Public Health 2008; 5: 139–146.

    Article  CAS  Google Scholar 

  17. Egwurugwu JN, Nwafor A, Ezekwe S . Impacts of prolonged exposure to gas flares on some blood indices in humans in the Niger Delta Region, Nigeria. Arch Appl Sci Res 2013; 5: 98–104.

    CAS  Google Scholar 

  18. Ibrahim KS, Amer NM, El-Droshky EA, Emara AM, El-Fattah AE, Shahy EM . Hematological effects of benzene exposure with emphasis on muconic acid as biomarker in exposed workers. Toxicol Ind Health, (e-pub ahead of print; doi:10.1177/0748233712458141.

  19. Yasmin S, Afroz B, Hyat B, D'Souza D . Occupational health hazards in women beedi rollers in Bihar, India. Bull Environ Contam Toxicol 2010; 85: 87–91.

    Article  CAS  Google Scholar 

  20. Vaziri ND . Mechanisms of lead-induced hypertension and cardiovascular disease. Am J Physiol Heart Circ Physiol 2008; 295: 454–465.

    Article  Google Scholar 

  21. Okediran B, Samuel, Ajibola E, Soloman, Thomas F, Clara et al. Investigation on sub-chronic lead intoxication on blood indices of male rats. Global Veterianaria 2010; 4: 532–535.

    Google Scholar 

  22. Mugahi MN, Heidari Z, Sagheb HM, Barbarestani M . Effects of chronic lead acetate intoxication on blood indices of male adult rat. DARU 2003; 11: 147–151.

    CAS  Google Scholar 

  23. Golalipour MJ, Roshandel D, Roshandel G, Ghafari S, Kalavi M, Kalavi K . Effect of lead intoxication and D-penicillamine treatment on hematological indices in rats. Int J Morphol 2007; 25: 717–722.

    Google Scholar 

  24. Shaik AP, Jamil K . Polymorphisms in MGP gene and their association with lead toxicity. Toxicol Mech Methods 2009; 19: 209–213.

    Article  CAS  Google Scholar 

  25. Shaik AP, Jamil K . A study on the ALAD gene polymorphisms associated with lead exposure. Toxicol Ind Health 2008; 24: 501–506.

    Article  CAS  Google Scholar 

  26. Gasparyan AY, Ayvazyan L, Mikhailidis DP, Kitas GD . Mean platelet volume: a link between thrombosis and inflammation? Curr Pharmaceutical Des 2011; 17: 47–58.

    Article  CAS  Google Scholar 

  27. Vagdatli E, Gounari E, Lazaridou E, Katsibourlia E, Tsikopoulou F, Labrianou I . Platelet distribution width: a simple, practical and specific marker of activation of coagulation. Hippokratia 2010; 14: 28–32.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Babu E, Basu D . Platelet large cell ratio in the differential diagnosis of abnormal platelet counts. Indian J Pathol Microbiol 2004; 47: 202–205.

    CAS  PubMed  Google Scholar 

  29. Biino G, Balduini C, Casula L, Cavallo P, Vaccargiu S, Parracciani D et al. Analysis of 12,517 inhabitants of a Sardinian geographic isolate reveals that predispositions to thrombocytopenia and thrombcytosis are inherited traits. Haematologica 2011; 96: 96–101.

    Article  Google Scholar 

  30. Samocha-Bonet D, Justo D, Rogowski O, Saar N, Abu-Abeid S, Shenkerman G et al. Platelet counts and platelet activation markers in obese subjects. Mediators Inflamm 2008; 83: 41–53.

    Google Scholar 

  31. Farhangi MA, Keshavarz SA, Eshraghian M, Ostadrahimi A, Saboor-Yaraghi AA . White blood cell count in women: relation to inflammatory biomarkers, hematological profiles, visceral adiposity, and other cardiovascular risk factors. J Health Popul Nutr 2013; 31: 58–64.

    Article  Google Scholar 

  32. Ballard Harold S . The hematological complications of alcoholism. Alcohol Health Res World 1997; 21: 42–52.

    CAS  PubMed  Google Scholar 

  33. Akanni EO, Mabayoje VO, Zakariyahu TO, Oparinde DP . Hematological characterization among heavy consumers in Oshogbo metropolis. Res J Med Sci 2010; 4: 48–52.

    Google Scholar 

  34. Gitte RN . Effects of cigarette smoking on plasma fibrinogen and platelet count. Asian J Med Sci 2011; 2: 181–184.

    Article  Google Scholar 

  35. Suwansaksri J, Wiwanitkit V, Soogarun S . Effect on smoking on platelet count and platelet parameters: an observation. Clin Appl Thrombosis/Hemostatis 2004; 10: 287–288.

    Article  Google Scholar 

  36. Dounias G, Rachiotis G, Hadjichristodoulou C . Acute lead intoxication in female battery workers: Diagnosis and management. J Occup Med Toxicol 2010; 5: 1–4.

    Article  Google Scholar 

  37. Balali-Mood M, Shademanfar S, Rastegar Moghadam J, Afshari R, Namaei Ghassemi M, Allah Nemati H et al. Occupational lead poisoning in workers of traditional tile factories in mashhad, northeast of Iran. Int J Occup Environ Med 2010; 1: 29–38.

    CAS  PubMed  Google Scholar 

  38. Adeyemo OK . Hematological profile of clarias gariepinus (Burchell, 1822) exposed to lead Turkish J Fisheries Aquatic Sci 2007; 7: 163–169.

    Google Scholar 

  39. Adeyemo OK, Adedeji OB, Offor CC . Blood lead level as biomarker of environmental lead pollution in feral and cultured African catfish (clarias gariepinus). Nigerian Veterinary J 2010; 31: 139–147.

    Google Scholar 

  40. Kianoush S, Balali-Mood M, Mousavi SR, Shakeri MT, Dadpour B, Moradi V et al. Clinical, toxicological, biochemical, and hematologic parameters in lead exposed workers of a car battery industry. Iran J Med Sci 2013; 38: 30–37.

    PubMed  PubMed Central  Google Scholar 

  41. Theron AJ, Tenting GR, Anderson R . Harmful interactions of non-essential heavy metals with cells of the innate immune system. J Clinic Toxicol 2012; S3: 1–10.

    Google Scholar 

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  1. Regional Occupational Health Centre (southern), Biochemistry, ICMR, Bangalore, India

    Tapu Barman, Ravibabu Kalahasthi & H R Rajmohan

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  1. Tapu Barman
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Correspondence to Tapu Barman.

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Barman, T., Kalahasthi, R. & Rajmohan, H. Effects of lead exposure on the status of platelet indices in workers involved in a lead-acid battery manufacturing plant. J Expo Sci Environ Epidemiol 24, 629–633 (2014). https://doi.org/10.1038/jes.2014.4

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