FIGURE 4

NOX inhibition alleviated the progress of cerebral postischemic inflammation. (A) Western blot analysis of intercellular adhesion molecule-1 (ICAM-1) expression after I/R. I/R induced ICAM-1 upregulation in the mouse brains. Con, contralateral; Ipsi, ipsilateral. (B) Summarized data from panel A are presented as a ratio of ICAM-1/-actin, which are normalized to the contralateral side. (^* P<0.05 versus wild-type (WT) mice). (C) ELISA analysis of myeloperoxidase (MPO) content in WT, apocynin-treated (Apo), and in gp91 knockout (KO) mice. In the ipsilateral hemisphere, MPO concentration was significantly higher in the WT group than in the apocynin-treated or gp91 KO mice (^* P<0.05 versus WT ipsilateral side, n=4 to 6). (D) Western blot analysis of cyclooxygenase-2 (COX-2) expression after I/R. COX-2 expression increased in the mouse brains after I/R. (E) Summarized data from panel D are presented as a ratio of COX-2/-actin, which are normalized to the contralateral side. There were no significant differences in COX-2 expression among the WT, apocynin-treated, and gp91 KO mice after I/R (P>0.05, n=3 to 5).