FIGURE 1

Infarct area is 2.5- to 3.5-fold larger in GFAP ^–/– Vim ^–/– (GV) than in WT mice 7 days after MCA transection. The infarct area was visualized by triphenyltetrazolium chloride and hematoxylin/erythrosin staining 7 days after proximal (A) and distal (C) transection of the MCA. (B and D) Infarct volume was more than two-fold larger in GFAP ^–/– Vim ^–/– than in WT mice (B, ^* P<0.05; D, ^*** P<0.001 and ^** P<0.01). (D) In GFAP ^–/– (G) and Vim ^–/– (V) mice, the infarct volume was not significantly different from that in WT mice. (E) Proximal MCA transection was performed at point X after bipolar coagulation at points A and B. Distal MCA transection was performed at point C (approximately 1.5 mm distal to point A). Values are means.e.m.