Original Article

Journal of Cerebral Blood Flow & Metabolism (2008) 28, 1656–1664; doi:10.1038/jcbfm.2008.57; published online 11 June 2008

Cerebral blood flow response in adenosine 2a receptor knockout mice during transient hypoxic hypoxia

This work was supported by a grant from the NIH: R0-1, NS-21076 (25) to HRW.

Greg Miekisiak1, Tobias Kulik1, Yoshikazu Kusano1, David Kung1, Jiang-Fan Chen2 and H Richard Winn1

  1. 1Department of Neurosurgery, Mount Sinai Medical School, New York, New York, USA
  2. 2Department of Neurology, Boston University School of Medicine, Boston, Massachusetts, USA

Correspondence: Professor Dr HR Winn, Department of Neurosurgery and Neuroscience, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1136, New York, NY 10029, USA. E-mail: richard.winn@mountsinai.org

Received 2 January 2008; Revised 30 April 2008; Accepted 8 May 2008; Published online 11 June 2008.

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Abstract

We evaluated cerebral blood flow by laser Doppler during 30 secs of hypoxia (0.10 FiO2) in anesthetized, ventilated adenosine 2a receptor knockout (A2aR KO) and wild-type (WT) mice to test the hypothesis that cerebral hypoxic hyperemia in KO mice would be attenuated. We also studied the effects of selective and nonselective A2aR antagonists. During 30 secs of hypoxia, PaO2 decreased significantly (P<0.05) and to a similar degree in both types of mice, whereas PaCO2 remained relatively stable. However, mean arterial blood pressure (MABP) decreased to a greater extent (P<0.05) during hypoxia in KO mice (58.6plusminus1.5 mm Hg) than in WT animals (76.1plusminus3.2 mm Hg). Consequently, in a separate group of mice, we stabilized and matched MABP during hypoxia. Hypoxic hyperemia was attenuated by 38% (P<0.05) in KO animals whose MABP was uncontrolled, and by 81% (P<0.05) in KO animals whose MABP changes were matched to the MABP in the hypoxic WT mice. In animals treated with adenosine antagonists, hypoxic hyperemia was decreased by 44% to 48% (P<0.05) in WT mice, but was without effect in KO mice. We conclude that adenosine via A2aR is responsible for a significant proportion of the hyperemia during hypoxia.

Keywords:

adenosine, CBF, hypoxia, knockout mice

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