FIGURE 6
FROM:
White matter injury correlates with hypertonia in an animal model of cerebral palsy
Alexander Drobyshevsky, Matthew Derrick, Alice Mary Wyrwicz, Xinhai Ji, Ila Englof, Lauren Marie Ullman, Mario Enrique Zelaya, Frances Josephine Northington and Sidhartha Tan
BACK TO ARTICLEFigure 6.
Staining for phosphorylated neurofilaments SMI312 at the level of the anterior thalamus shows dramatic reduction in hypoxic animals with hypertonia (right sided panels) in the corpus callosum (CC, panel B) and internal capsule (IC, panel D), relative to controls (A and C, correspondingly). There is a loss of immunoreactivity for phosphorylated neurofilaments in hypertonia group, shown with brown DAB precipitate, in both the CC (B) and IC (D). Microphotographs of corpus callosum with higher magnification (E-controls, F-hypoxia with hypertonia), show white matter fibers to be less dense, less compact and less streamlined in hypertonic animals, which can explain the reduction of FA and increase of radial diffusivity in white matter (see Figure 4C and 1A). Decrease in optical density of the neurofilaments staining was significant in CC and IC of hypertonic animals (*, P<0.05, Mann–Whitney test). Scale bar is 1 mm.
