FIGURE 3
FROM:
Endotoxin preconditioning protects against the cytotoxic effects of TNF
after stroke: a novel role for TNF in LPS-ischemic tolerance
Holly L Rosenzweig, Manabu Minami, Nikola S Lessov, Sarah C Coste, Susan L Stevens, David C Henshall, Robert Meller, Roger P Simon and Mary P Stenzel-Poore
BACK TO ARTICLEFigure 3.
Soluble TNF
is essential for LPS preconditioning in an in vitro model of ischemia. (A) Cortical neuronal cultures were pretreated with 1 
g/mL LPS for 24 h before exposure to OGD. Percentage cell death was determined 24 h after OGD by PI staining. (B) TNF activity present in the cell culture supernatant after LPS treatment alone measured by WEHI assay. (C) Cortical cultures were cotreated with anti-TNF neutralizing antibody (3 g/mL) and LPS for 24 h before OGD, and % cell death was examined 24 h after OGD as measured by PI staining. Anti-TNF antibody or control immunoglobulin G treatment at 24 h before OGD had no effect on cell viability or OGD-induced cell death (data not shown). (D) Cortical cultures were cotreated with TAPI (TACE inhibitor, 8 mol/L) and LPS for 24 h before OGD, and % cell death was determined 24 h later by PI staining. For all experiments, values are mean
s.e.m., *
P<0.05 versus media-treated OGD controls, n=4 to 6 individually repeated experiments.
