FIGURE 2

Temporal profile of bone marrow-derived Iba-1/EGFP-positive monocytes/macrophages and intrinsic microglia/macrophages after brain ischemia/reperfusion. (A) Schematic representation of the distribution of neuronal damage in mice brain after reperfusion delineated by loss of MAP-2 staining. The shaded area represents the infarct zone. Three areas subjected to immunohistochemical analysis are illustrated. Pe: periinfarct area; Tr: transition area; Co: ischemic core area. (B) Iba-1 staining of control (a–c) and G-CSF (d–f) groups at 24 h (a, d), 72 h (b, e), and 7 days (c, f) in the Tr area. Note the prominent microglial activation in the G-CSF group. Scale bar=50 m. (C) Photomicrographs showing Iba-1/EGFP double immunofluorescence staining of control (a–c) and G-CSF (d–f) groups at 24 h (a, d), 72 h (b, e), and 7 days (c, f) after reperfusion. Intrinsic microglia/macrophages appeared red while extrinsic monocytes/macrophages appeared yellow with colocalization of Iba-1 and EGFP expression. The remaining green fluorescent cells were identified as leukocytes. Scale bar=100 m. (D) Numbers of extrinsic (Ex) activated monocytes/macrophages and intrinsic (In) activated microglia/macrophages were counted in each parietal cortex including the Pe area (a), Tr area (b), and Co area (c). Data are means.e.m. of five mice in each group. ^* P<0.001, compared with the corresponding control group.