FIGURE 4
FROM:
Prevention of apoptosis-inducing factor translocation is a possible mechanism for protective effects of hepatocyte growth factor against neuronal cell death in the hippocampus after transient forebrain ischemia
Makiko Niimura, Norio Takagi, Keiko Takagi, Reiko Mizutani, Naoko Ishihara, Kunio Matsumoto, Hiroshi Funakoshi, Toshikazu Nakamura and Satoshi Takeo
BACK TO ARTICLEFigure 4.
Effect of hepatocyte growth factor (HGF) on phosphorylation of the N-methyl-D-aspartate (NMDA) receptor in the hippocampal cornu ammonis (CA) subregion 1 (CA1) region after transient forebrain ischemia. Proteins (50 
g) from nonoperated naïve rats and four-vessel-occluded rats at 1, 6, and 24 h of reperfusion without (I/R) or with HGF (I/R+HGF) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and subjected to immunoblotting with anti-phospho-NR2B antibody [pY1472 NR2B]. The blots were then stripped and reprobed with anti-NR2B antibody [NR2B]. Bands corresponding to pY1472 NR2B or NR2B were scanned, and the scanned bands of pY1472 NR2B were normalized by NR2B on the same blot. Results are the mean percentages of the nonoperated naïve control
s.e. n=3. *
P<0.05 versus nonoperated naïve control, analysis of variance (ANOVA) with post hoc Fischer's protected least significant difference.