FIGURE 3
FROM:
Release of bradykinin and expression of kinin B2 receptors in the brain: role for cell death and brain edema formation after focal cerebral ischemia in mice
Moritz Gröger, Diane Lebesgue, Didier Pruneau, Jane Relton, Seong-Woong Kim, Jürg Nussberger and Nikolaus Plesnila
BACK TO ARTICLEFigure 3.
Immunohistochemistry for bradykinin B2 receptors in the healthy brain and at different time points after 45 mins of MCAO in wild-type mice. No staining is observed in infarcted tissue (E) or when the primary antibody was omitted (A). B2 receptors are only expressed on cortical cells with neuronal morphology (B). Healthy cells show a nuclear staining pattern (B). After ischemia, cells showing signs of ischemic damage change their staining from nuclear to a more membrane-related pattern (C, arrows). Twenty-four hours after MCAO, most cells in infarcted tissue do not show any B2 receptor staining (E), while most cells in the margin of the infarct have marked B2 receptor positive cell membranes (F). Panels A, E, and F were taken using a 
20 objective. Panels B−D were taken using a 40 objective, and the high-power insets in panels B and C were photographed using a 100 oil-immersion objective.