High-resolution blood|[ndash]|brain barrier permeability and blood volume imaging using quantitative synchrotron radiation computed tomography: study on an F98 rat brain glioma

doi:doi:10.1038/sj.jcbfm.9600017

Journal of Cerebral Blood Flow & Metabolism homepage

Journal of Cerebral Blood Flow & Metabolism (2005) 25, 145–153. doi:10.1038/sj.jcbfm.9600017

High-resolution blood–brain barrier permeability and blood volume imaging using quantitative synchrotron radiation computed tomography: study on an F98 rat brain glioma

Jean-François Adam¹, Christian Nemoz², Alberto Bravin², Stefan Fiedler², Sam Bayat², Sylvie Monfraix², Gilles Berruyer², Anne Marie Charvet¹, Jean-François Le Bas^1,3, Hélène Elleaume¹ and François Estève^1,3

¹Unité INSERM 647 RSRM (Rayonnement Synchrotron et Recherche Médicale), ID17-ESRF, Grenoble, France
²Medical Beamline ID17, European Synchrotron Radiation Facility (ESRF), Grenoble, France
³Unité IRM, Centre Hospitalo-Universitaire de Grenoble, Grenoble, France

Correspondence: Dr F Estève, Unité INSERM 647 RSRM, Medical Beamline ID17, ESRF, 6, rue Jules Horowitz, BP220, 38043 Grenoble Cedex, France. E-mails: esteve@esrf.fr, FEsteve@chu-grenoble.fr

Received 9 February 2004; Revised 2 August 2004; Accepted 15 October 2004.

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Abstract

The authors previously provided evidence of synchrotron radiation computed tomography (SRCT) efficacy for quantitative in vivo brain perfusion measurements using monochromatic X-ray beams. However, this technique was limited for small-animal studies by partial volume effects. In this paper, high-resolution absolute cerebral blood volume and blood–brain barrier permeability coefficient measurements were obtained on a rat glioma model using SRCT and a CCD camera (47 $times$ 47 m² pixel size). This is the first report of in vivo high-resolution brain vasculature parameter assessment. The work gives interesting perspectives to quantify brain hemodynamic changes accurately in healthy and pathological small animals.