Journal of Cerebral Blood Flow & Metabolism

FIGURE 5

FROM:

Ischemia-Induced Brain Damage Depends on Specific Gap-Junctional Coupling

Marina V Frantseva, Larisa Kokarovtseva and Jose L Perez Velazquez

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Figure 5.

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Reduction of GJC by antisense treatment results in decreased postischemic cell death. (A and B) The PI fluorescence in a slice incubated in the presence of the missense oligodeoxynucleotide (ODN) (30 mumol/L) 24 and 48 hours after the hypoxic−hypoglycemic episode. (C−F) Two slices incubated in the presence of antisense ODNs for Cx26 and Cx32 simultaneously (30 mumol/L) at 24 and 48 hours, (C and E) 24 hours after the ischemic insult, and (D and F) after 48 hours. Note the reduced PI fluorescence. Same scale as Fig. 1. For quantitative results, see Fig. 7.

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