FIGURE 2
FROM:
Cerebral Ischemia Produces Laddered DNA Fragments Distinct From Cardiac Ischemia and Archetypal Apoptosis
John P MacManus, Henry Fliss, Edward Preston, Ingrid Rasquinha and Ursula Tuor
BACK TO ARTICLEFigure 2.
Ligation-mediated PCR demonstrates that the majority of laddered DNA fragments from ischemic adult brain are not blunt ended. Fivefold serial dilutions of target DNA (1000−8 pg DNA) purified from dexamethasone-treated thymic lymphocytes, striatum 48 hours after two-vessel occlusion, cortex 24 hours after middle cerebral artery occlusion, and ischemic heart with 4 hours of reperfusion were subjected to PCR with and without prior Klenow polymerase treatment. (A) The majority of DNA fragments from apoptotic T lymphocytes are blunt ended and show no effect of polymerase pretreatment. (B and C) The majority of DNA fragments from ischemic brain are not blunt ended, but pretreatment with polymerase permits subsequent amplification. (D) The DNA fragments from ischemic myocardium are predominantly blunt ended and do not benefit from polymerase pretreatment.
