FIGURE 4

Gel electrophoresis of polymerase chain reaction (PCR) products for the NPY-Y1, NPY-Y2, and NPY-Y4 receptors amplified from reverse transcribed DNA (+) from human pial vessels (PV), isolated intracortical microvessels (MV) and capillaries (CAP), as well as from human microvascular smooth muscle (SMC), endothelial (END), and astroglial (AST) cells in culture. Expression of the Y1 receptor was detected in every segment of the human cerebrovascular bed and cells in culture except for the END cells. Expression of Y5 receptor mRNA could not be detected in any vascular or astroglial preparations, despite a positive and specific amplification in human cerebral cortex (not shown). Samples without reverse transcriptase were included (-) to monitor for background amplification and possible contamination.