Original Article

The Journal of Antibiotics (2012) 65, 473–477; doi:10.1038/ja.2012.46; published online 13 June 2012

3′-Demethyldihydromaldoxin and dihydromaldoxin, two anti-inflammtory diaryl ethers from a Steganospora species

Dominik Schreiber1, Mathias Jung2, Louis P Sandjo3, Johannes C Liermann3, Till Opatz3 and Gerhard Erkel1

  1. 1Department of Molecular Biotechnology and Systems Biology, University of Kaiserslautern, Kaiserslautern, Germany
  2. 2Institute of Biotechnology and Drug Research (IBWF), Kaiserslautern, Germany
  3. 3Institute of Organic Chemistry, University of Mainz, Mainz, Germany

Correspondence: Dr G Erkel, Department of Molecular Biotechnology and Systems Biology, University of Kaiserslautern, Paul-Ehrlich-Strasse 23, D-67663 Kaiserslautern, Germany. E-mail: erkel@rhrk.uni-kl.de

Received 1 February 2012; Revised 23 April 2012; Accepted 15 May 2012
Advance online publication 13 June 2012

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Abstract

CXCL10 (IP-10) is a highly inducible chemoattractant, which contributes to the recruitment of inflammatory cells such as macrophages and T-lymphocytes and thereby has important roles in chronic inflammatory conditions. In a search for new inhibitors of CXCL10 expression in MonoMac6 (MM6) cells, the new diaryl ether 3′-demethyldihydromaldoxin (1) along with the known compound dihydromaldoxin (2), were isolated from fermentations of a Steganospora species. The structures of the compounds were elucidated by a combination of one- and two-dimensional NMR spectroscopy and mass spectrometry. Compounds (1) and (2) inhibited lipopolysaccharide (LPS)/interferon-γ (IFN-γ)-induced CXCL10 promoter activity in transiently transfected MM6 cells in a dose-dependent manner with IC50 values of 39–41μM and also reduced LPS/IFN-γ-induced CXCL10 protein synthesis and excretion.

Keywords:

CXCL10; 3′-demethyldihydromaldoxin; inhibitor

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Introduction

Chemokine (C-X-C motif) ligand 10 (CXCL10)/interferon-γ (IFN-γ)-inducible protein 10 (IP-10) is a small molecule that is secreted mainly by monocytes, fibroblasts and endothelial cells in response to diverse stimuli, such as IFN-γ, lipopolysaccharide (LPS), interleukin-1β (IL-1β) and viral infection. The biological function of CXCL10 involves the recruitment of monocytes, macrophages and T cells to sites of inflammation. CXCL10 also participates in the regulation of T-cell responses due to modulation of T-cell proliferation in response to an antigenic challenge and favoritism of Th1-type cytokine production while downregulating Th2 cytokines. Although essential for effective host defense against infections, an increased expression of CXCL10 may also contribute to the induction and exacerbation of chronic inflammatory reactions associated to graft rejection or allergic and autoimmune diseases such as asthma, chronic obstructive pulmonary disease, acute inflammation in response to pathogens, inflammatory bowel diseases as well as rheumatoid arthritis.1, 2 Therefore, CXCL10 might be an attractive target for the development of new therapeutics against various chronic inflammatory and autoimmune diseases.3 A screening of fungal extracts inhibiting LPS/IFN-γ-induced CXCL10 promoter-dependent reporter gene activity in transiently transfected MonoMac6 (MM6) cells, led to the isolation of the new diaryl ether 3′-demethyldihydromaldoxin (1) along with its known relative dihydromaldoxin (2) from fermentations of the fungus Steganospora sp. Diaryl ethers are found as common metabolites in marine sponges, lichens, plants and fungi. Dihydromaldoxin, a chlorinated derivative of asterric acid, as well as various other chlorinated diaryl ethers have been isolated from different members of the genus Aspergillus, Penicillium, Xylaria and Pestalotiopsis.4, 5, 6 Asterric acid has been described to be the first non-peptidic inhibitor of the endothelin receptor and dechlorodihydromaldoxin and dihydromaldoxin were found to selectively inhibit endothelin-1 binding to endothelin type A receptor with IC50 values of 1.5 and 20μM, respectively.7, 8 These compounds also inhibit the increase in intracellular Ca2+ concentration elicited by 1nM endothelin-1 in A10 cells.8 In addition, the asterric acid derivatives methyl asterric acid, methyl 3-chloroasterric acid and methyl 3,5-dichloroasterric acid exerted inhibitory activity on VEGF-induced capillary-like tube formation of human umbilical vein endothelial cells in vitro.9 Herein, we describe the fermentation, structure elucidation, and anti-inflammatory activities of 3′-demethyldihydromaldoxin (1) in comparison with dihydromaldoxin (2).

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Results and Discussion

Identification and structure elucidation

3′-Demethyldihydromaldoxin (1) and dihydromaldoxin (2) (Figure1) were isolated from the culture fluid extract by bioactivity-guided fractionation as described in the experimental section.

Figure 1.
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Structures of Compounds (1) and (2).

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Compound (1) was obtained as yellowish solid and its molecular formula C16H13ClO8 was determined on the basis of HR-ESI-MS which gave a pseudo-molecular ion at m/z 391.0199 (calculated 391.0191) [M+Na]+. This formula requires 10 degrees of unsaturation, and the presence of chlorine was reflected by the characteristic isotope pattern. The IR spectrum showed bands for a carboxylic acid function (3496, 1692cm–1), an ester group (1683cm–1) and an aromatic ring (1579cm–1). The 1H- and 13C-NMR spectra (Table 1) exhibited resonances attributable to one methyl, one methoxy and three aromatic methine groups. Analysis of diagnostic HMBC correlations and the comparison of the chemical shifts with those of asterric acid8 indicated the presence of two benzoic acid moieties linked in form of a diaryl ether and suggested a close structural similarity to dihydromaldoxin. Thus, some neighboring and long-range correlations (Figure 2) were detected in the HMBC spectrum from H-3 (δ 6.56) to C-1 (δ 100.9), C-2 (δ 164.7), C-5 (δ 106.3), C-7 (δ 171.7) and C-8 (δ 21.9). The same feature was observed between H-5 (δ 6.01) and the carbons C-1 (δ 100.9), C-3 (δ 113.7), C-4 (δ 148.6), C-6 (δ 158.0), C-7 (δ 171.7) and C-8 (δ 21.9). Further cross peaks between H-2′ (δ 7.41) and the carbons C-1′ (δ 124.1), C-3′ (δ 149.1), C-4′ (δ 113.5), C-5′ (δ 146.6), C-6′ (δ 139.4), and C-7′ (δ 170.3) showed the difference between 1 and asterric acid to be the presence of a chlorine at C-4′ of compound (1); besides, the presence of a hydroxyl function at C-3′ instead of a MeO group distinguishes its structure from that of dihydromaldoxin. The NOESY contact between the methoxy protons at δ 3.95 and H-2′ (δ 7.41) permitted the location of the ester function at C-7′, the aromatic methyl group exhibited only trivial NOE contacts to both neighboring protons.

Figure 2.
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COSY and HMBC correlations of compound (1).

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Compound (2) was described before as RES-1214-2,8 dihydromaldoxin5 and pestheic acid.10

Biological activities

In order to identify active compounds and to characterize their influence on CXCL10 expression, we used a human CXCL10 promoter-dependent transcriptional reporter in transiently transfected monocytic MM6 (MM6) cells, which reflects the activation of the transcription factors binding to the regulatory sites of the CXCL10 gene.11 Cells of the monocytic system (macrophages, monocytes) form a central part in immune defense by presenting processed antigen to T cells, phagocytosis of foreign material and orchestrating the immune response by secretion of cytokines. Typically, these cells, when triggered by an immunogen, produce proinflammtory cytokines (for example, tumor necrosis factor-α, interleukin-1β, interleukin-6), chemokines (for example, CXCL10, CXCL8) and proinflammatory enzymes (for example, COX-2).12 Transfection of MM6 cells with a hCXCL10 promoter-driven luciferase reporter plasmid and stimulation with 1μgml−1 LPS and 10ngml−1 IFN-γ increased luciferase activity 8–10 fold compared with non-stimulated cells. As shown in Figure 3a, the inducible promoter activity was dose dependently inhibited by 3′-demethyldihydromaldoxin (1) and dihydromaldoxin (2) with IC50 values of 41 and 39.2μM (15μgml−1), respectively. The constitutive activity of the EF-1α-promoter-driven expression of the reporter gene renilla luciferase was not significantly affected up to a concentration of 50μgml−1 of compound (1) and (2) (135 and 131μM, respectively) indicating that the compounds do not interfere with transcription in a general manner. The NF-κB pathway has been shown to be an essential modulator of the transcription of chemokine genes such as CXCL10.13, 14 We therefore investigated the effect of (1) and (2) on NF-κB-driven expression of the reporter gene luciferase in LPS/IFN-γ-stimulated MM6 cells. As shown in Figure 3b, both compounds inhibited the NF-κB-dependent expression of the reporter gene with IC50 values around 27μM (10μgml−1). In addition to NF-κB, the response of the 972bp CXCL10 promoter construct largely depends on three distal interferon-stimulated response elements (ISRE) and two Stat recognition sites.15 We therefore investigated the effect of 1 and 2 on Stat1 and ISRE-driven expression of the reporter gene luciferase in LPS/IFN-γ-stimulated MM6 cells. As shown in Figure 3c, the ISRE-dependent expression of the reporter gene was inhibited by both compounds with IC50 values of 59μM dihydromaldoxin (2) and 90μM 3′-demethyldihydromaldoxin (1), whereas the Stat1-dependent transcriptional reporter was not significantly affected.

Figure 3.
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Effect of (1) and (2) on CXCL10 promoter activity, NF-κB-, Stat1- and ISRE-driven reporter gene expression. (a)MonoMac6 (MM6) cells were transiently transfected with a CXCL10- or EF1α-promoter-dependent reporter construct and stimulated with 1μgml−1 lipopolysaccharide (LPS) and 10ngml−1 IFN-γ for 4h with or without test compounds. Control (100%): stimulation only. Results represent the mean±s.e.m. of at least three independent experiments. (b) MM6 cells were transiently transfected with a NF-κB-dependent reporter construct and stimulated with 1μgml−1 LPS and 10ngml−1 IFN-γ for 4h with or without test compounds. Control (100%): stimulation only. Results represent the mean±s.e.m. of at least three independent experiments. (c) MM6 cells were transiently transfected with an ISRE-or GAS-dependent reporter construct and stimulated with 1μgml−1 LPS and 10ngml−1 IFN-γ for 4h with or without test compounds. Control (100%): stimulation only. Results represent the mean±s.e.m. of at least three independent experiments. The expression of the reporter genes was determined as described in the experimental section.

Full figure and legend (123K)

To investigate the effect of 3′-demethyldihydromaldoxin (1) and dihydromaldoxin (2) on the inducible transcription of the CXCL10 gene, quantitative real-time PCR experiments were performed with total RNA isolated from cells stimulated with LPS/IFN-γ for 4h and treated with different concentrations of test compounds as described in the Materials and methods section. Values are expressed as relative mRNA content of induced versus uninduced cells (100%), and induced and compound treated versus induced and untreated cells, each corrected for the constitutive expressed housekeeping gene GAPDH as reference determined in the same sample in parallel. As shown in Figure 4, stimulation of MM6 cells with LPS and IFN-γ resulted in a strong upregulation (~1250-fold; 100%) of the mRNA level for CXCL10. Uninduced cells did not produce detectable amounts of CXCL10 mRNA. At a concentration of 26.2μM, dihydromaldoxin (2) significantly downregulated the mRNA level of CXCL10 in LPS/IFN-γ-induced MM6 cells around 50%, whereas 3′-demethyldihydromaldoxin (1) reduced CXCL10 mRNA level to a lesser extent. The measured mRNA values for GAPDH did not vary significantly upon treatment of the cells with LPS/IFN-γ or test compounds (data not shown).

Figure 4.
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Effect of (1) and (2) on mRNA levels of CXCL10 in LPS/IFN-γ-stimulated MonoMac6 (MM6) cells. Values represent the relative mRNA levels of stimulated versus unstimulated cells as control (100%) and compound pretreated and stimulated versus untreated, stimulated cells, corrected for GAPDH as reference determined in the same sample in parallel. Data are shown as mean values±s.e.m. of three independent experiments.

Full figure and legend (39K)

In turn, we analyzed the influence of (1) and (2) on CXCL10 synthesis and excretion in MM6 cells, which release significant amounts of CXCL10 following LPS/IFN-γ treatment. As shown in Figure 5, pretreatment of MM6 cells with various concentrations of both compounds and stimulation with LPS/IFN-γ for 16h resulted in a dose-dependent inhibition of CXCL10 synthesis, which was comparable to the results obtained in the quantitative real-time PCR assays. The above-mentioned results indicate that the fungal metabolites exert their inhibitory activities on the transcriptional level and thus results in a reduced protein level.

Figure 5.
Figure 5 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Effect of (1) and (2) on CXCL10-production and cell viability. MonoMac6 (MM6) cells were pretreated for 1h with or without the test compounds and stimulated with 1μgml−1 lipopolysaccharide (LPS) and 10ngml−1 IFN-γ for additional 24h. CXCL10 concentrations in cell supernatants were determined by ELISA. Cell viability was determined by XTT colorimetric assay. Data are shown as mean values±s.e.m. of three independent experiments.

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Cytotoxic properties of (1) and (2) were evaluated against MM6 cells by measuring the reduction of the tetrazolium compound 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium (XTT) into a colored formazan, which is directly proportional to the number of living cells in culture. No significant cytotoxic activities against MM6 cells could be observed up to 50μgml−1 (131–135μM) of both compounds during a 24-h incubation period. In addition, both compounds exhibited no antibacterial or antifungal activities up to a concentration of 50μg per disc in an agar diffusion assay.

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Materials and methods

General procedures

1H NMR (400MHz) and 13C NMR (101MHz) were recorded with a Bruker Avance-II spectrometer (Bruker Daltonik, Bremen, Germany) equipped with a 5-mm BBO probe head. The spectra were measured in CDCl3 and the chemical shifts were referenced to the residual solvent signal (δH=7.26p.p.m., δC=77.16p.p.m.).16 APCI-MS spectra were measured from a solution of the analyte in MeCN/H2O with a Hewlett Packard MSD 1100 (Agilent Technologies, Waldbronn, Germany) using an evaporator temperature of 400°C, a drying gas temperature of 350°C at a flow of 6l per h (N2). In positive ionization mode, the capillary voltage amounted to 3.5kV, the corona discharge current was 4μÅ. In negative ionization mode, the capillary voltage amounted to 2.2kV, the corona discharge current was 6μÅ. ESI-HRMS data were measured from a solution of the analyte in acetonitrile with a Waters Q-TOF-Ultima 3 (Waters GmbH, Eschborn, Germany) equipped with a LockSpray interface (tri-n-octylamine as external reference). IR and UV spectra were measured with a Bruker IFS48 FTIR spectrometer (Bruker Daltonik) and a Perkin-Elmer Lambda-16 spectrophotometer (Perkin-Elmer, Rodgau, Germany), respectively. The optical rotation was measured on a Perkin-Elmer 241 polarimeter at 578 and 546nm and extrapolated to 589nm using Drude’s equation.

Culturing and isolation of metabolites

Steganospora sp. strain IBWFE07110 was isolated from a twig of Robinia pseudoacacia collected in Germany. A three-step ethanol, sodium hypochlorite and ethanol treatment was used to isolate the endophytic fungus. The strain is deposited in the culture collection of the Institute of Biotechnology and Drug Research (IBWF e.V.), Kaiserslautern, Germany. Steganospora sp. strain IBWFE07110 showed all characteristics of the genus, the species, however, could not be unequivocally determined. Owing to the lack of similar sequences, identification of the strain to the species level by ITS sequence analysis of the ITS1-5.8S rDNA-ITS2 region of nuclear DNA17 was not possible. For maintenance, the fungus was grown on HA agar slants (yeast extract 4gl−1, malt extract 10gl−1, glucose 4gl−1; the pH value was adjusted to 5.5 before autoclaving). Solid media contained 2% of agar. Well-grown seed cultures of the producing strain (200ml HA medium) were used to inoculate a Biolafitte C-6 fermenter containing 20l of malt extract medium (20gl−1 malt extract, pH 5.5) with aeration (3.0l airmin−1) and stirring (120r.p.m.) at 22°C. The production of compounds (1) and (2) was detected by the inhibitory effect of various concentrations of a crude extract of the culture fluid in the CXCL10 promoter-dependent reporter gene assay as described below. The compounds were isolated from the culture fluid by bioactivity-guided fractionation using the CXCL10 transcriptional reporter assay in MM6 cells.

After 306h, the culture fluid was separated from the mycelia by filtration, extracted twice with an equal volume of EtOAc and dried over Na2SO4. After concentration in vacuo, the crude extract (9.5g) was fractionated by subsequent chromatography on silica gel (Merck 60, eluent: cyclohexane : EtOAc, 40 : 60v/v). The enriched fraction (2.5g) was then chromatographed over silicic acid (SIL-A-200, Sigma-Aldrich, Taufkirchen, Germany) using cyclohexane/EtOAc gradient as the mobile phase. The enriched fraction (355.2mg) was further purified on a CHROMABOND C18 column (Macherey-Nagel, Düren, Germany) with a H2O/MeCN gradient to yield 210mg of enriched product. Further purification was achieved by semi-preparative HPLC on a phenyl reversed phase column (Zorbax Eclipse XDB Phenyl, 21.2 × 150mm2, 5μM, Agilent Technologies) using HCOOH/H2O (63% (0.3 vol% HCOOH): 37% MeCN; flow-rate 21mlmin−1) as eluent to obtain 23.1mg dihydromaldoxin (2) and 12.2mg 3′-demethyldihydromaldoxin (1).

3′-Demethyldihydromaldoxin (1): slightly yellow amorphous powder; Mp 222–224oC; ESI-MS: m/z 369 [M+H]+, 351 [M+H–H2O]+, 319 [M+H–CH3Cl]+; IR ν (cm–1): 3497, 2958, 2928, 1692, 1683, 1579; UV, λmax (logε, MeCN): 211 (4.40), 250 (3.99), 330 (3.61) nm, NMR data: see Table 1.

Biological assays

Cell culture and cytotoxicity assay
 

MM6 (DSMZ ACC124) cells were maintained in RPMI 1640 medium containing 25mM HEPES buffer, 2mM L-glutamine, supplemented with 10% FCS, 100uml−1 penicillin and 100μgml−1 streptomycin at 37°C and 5% CO2 in a humidified atmosphere. The cytotoxicity against MM6 cells was determined in an XTT-based cell viability assay.18 The assays for antimicrobial activity were carried out as described previously.19 Human recombinant IFN-γ (R&D Systems, Wiesbaden, Germany) and LPS (Sigma-Aldrich) was dissolved in RPMI 1640 medium containing 0.5% FCS at a final concentration of 10 and 1mgml−1, respectively. Stock solutions of the test compounds were prepared in EtOH and diluted into the cell culture medium to give a final concentration<0.1% of the solvent.

Reporter gene assays
 

The human IP-10 Promoter (−875 to +97) was amplified by PCR from genomic DNA extracted from MM6 cells. The PCR product was cloned into the pGL4-luc basic vector (Promega, Mannheim, Germany) to generate the firefly luciferase reporter plasmid pGL4-IP10pro. The plasmid pRL-EF-1α for normalizing transfection efficiency was obtained from Promega. The NF-κB-, Stat1- and IRF-driven reporter plasmids pNFκB-Luc, pGAS-Luc and pISRE-Luc were obtained from Clontech (Saint-Germain-en-Laye, France). Transient transfections of MM6 cells were performed by electroporating (BioRad, Munich, Germany; Gen Pulser) 3 × 107 cellsml−1 in 0.4ml RPMI 1640 medium containing 25mM HEPES buffer together with 40μg of the indicated plasmids at 200V and 975μF.20 After electroporation the cells were seeded at 1 × 106 cellsml−1 RPMI 1640 medium containing 10% FCS in a 24-well plate with and without test compounds and luciferase expression was induced with 1μgml−1 LPS and 10ngml−1 IFN-γ. Luciferase activity was measured 4h after induction using the Dual-Glo luciferase assay system (Promega) according to the manufacturer’s instructions with a luminometer.

Quantitative real-time PCR
 

For qRT–PCR analysis, MM6 cells were starved for 16h in RPMI 1640 medium with 0.5% FCS. Afterwards, the cells were plated into six-well plates at a density of 3 × 106 cells per well. After treatment with test compounds for 1h, the cells were induced with 1μgml−1 LPS and 10ngml−1 IFN-γ for 4h. Following stimulation, the cells were lysed and total cellular RNA was prepared by using the RNeasy mini kit obtained from Qiagen (Hilden, Germany). First strand cDNA was generated using the RevertAid H Minus First Strand cDNA Synthesis Kit (Fermentas, St Leon-Roth, Germany). Gene expression was quantified from 300ng cDNA using the 5x Hot Start Taq EvaGreen qPCR Mix (Axon, Kaiserslautern, Germany) according to manufacturer’s suggestions with gene-specific primers for human CXCL10 (Genbank Accession NM001565) forward: 5′-TGAGCCTACAGCAGAGGAA-3′ and reverse: 5′-TACTCCTTGAATGCCACTTAGA-3′ (size of the PCR product is 102bp) and GAPDH (Genbank Accession M33197) forward: 5′-CCTCCGGGAAACTGTGG-3′ and reverse: 5′-AGTGGGGACACGGAAG-3′ (size of the PCR product is 140bp). All primers were manufactured by MWG-Biotech AG (Ebersberg, Germany). Measurements were done using the CFX96 detection system (BioRad) with the following protocol: initial activation of HotStar Taq DNA polymerase at 95°C for 15min, 45 cycles of 94°C for 15s, annealing at 56°C for 30s, extension/detection at 72°C for 30s. Relative mRNA amounts were determined using the mathematical model for relative quantification in real-time PCR proposed by Pfaffl21 in which the relative expression ratio is calculated from the real-time PCR efficiencies and the crossing point deviation of an unknown sample versus glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as control housekeeping gene.

ELISA
 

For analyzing CXCL10 synthesis MM6 cells were starved for 16h in RPMI 1640 medium containing 0.5% FCS, pretreated for 1h with the test compound and subsequently induced with 1μgml−1 LPS and 10ngml−1 IFN-γ for additional 24h. Subsequently, the cells were centrifuged and the cell culture supernatant was analyzed using the CXCL10 ELISA kit (R&D Systems) according to the manufacturer’s instructions.

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Conclusion

In summary, we have identified the new diaryl ether 3′-demethyldihydromaldoxin (1) along with the known dihydromaldoxin (2) as inhibitors of CXCL10 expression from fermentations of a Steganospora species. Thus, diaryl ethers of this type may serve as a starting point for the development of transcriptionally based inhibitors of chronic inflammatory disorders.

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Conflict of interest

The authors declare no conflict of interest.

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Acknowledgements

This work was supported by a grant from the Stiftung Rheinland-Pfalz für Innovation and by the Naturstoffzentrum Rheinland-Pfalz. We thank A Meffert for expert technical assistance.