Original Article

The Journal of Antibiotics (2011) 64, 655–659; doi:10.1038/ja.2011.68; published online 3 August 2011

Khatmiamycin, a motility inhibitor and zoosporicide against the grapevine downy mildew pathogen Plasmopara viticola from Streptomyces sp. ANK313

Muna Ali Abdalla1, Hnin Yu Win1, Md. Tofazzal Islam2,3, Andreas von Tiedemann3, Anja Schüffler4 and Hartmut Laatsch1

  1. 1Institute of Organic and Biomolecular Chemistry, Georg-August University Göttingen, Göttingen, Germany
  2. 2Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh
  3. 3Department of Crop Sciences, Division of Plant Pathology and Plant Protection, Georg-August-University Göttingen, Göttingen, Germany
  4. 4Institute of Biotechnology and Drug Research (IBWF), Kaiserslautern, Germany

Correspondence: Professor H Laatsch, Institute of Organic and Biomolecular Chemistry, University of Goettingen, Tammannstrasse 2, Goettingen D-37077, Germany. E-mail: hlaatsc@gwdg.de

Received 15 December 2010; Revised 28 June 2011; Accepted 29 June 2011; Published online 3 August 2011.



In the course of our screening for anti-peronosporomycetal agents, we isolated a new compound khatmiamycin (1), together with five known metabolites, GTRI-02 (3), 4-ethyl-5-methyl-heptanamide (4), aloesaponarin II (5), LL-C10037α (6) and LL-C10037β (7) from the culture broth of a terrestrial Streptomyces sp. ANK313. The structures of these metabolites were assigned on the basis of their spectroscopic data. Khatmiamycin (1) exhibited potent motility inhibitory (100%) and lytic (83±7%) activities against zoospores of the grapevine downy mildew pathogen Plasmopara viticola at 10μgml−1, followed by compounds 5 (MIC 25μgml−1), 7, 6, 3 in the order of decreasing activity. Khatmiamycin (1) also showed potent antibacterial activity against Staphylococcus aureus and Streptomyces viridochromogenes (Tü57) by causing inhibition zones of 11 and 14mm diameter, respectively, at the dose of 40μg per disk. This is the first report on motility inhibitory and lytic activities of metabolites from a terrestrial Streptomyces species against the zoospores of downy mildew pathogen P. viticola.


antibacterial; naphthoquinones; oomycetes; Streptomyces; zoosporicide