1. ¹Department of Microbiology, IWWR, Radboud University Nijmegen, Toernooiveld 1, Nijmegen, The Netherlands
2. ²Department of Aquatic Ecology and Environmental Biology, IWWR, Radboud University Nijmegen, Toernooiveld 1, Nijmegen, The Netherlands

Correspondence: HJM Op den Camp, Department of Microbiology, IWWR, Radboud University Nijmegen, Toernooiveld 1, NL-6525 ED, The Netherlands. E-mail: H.opdenCamp@science.ru.nl

Received 15 May 2008; Revised 3 July 2008; Accepted 4 July 2008; Published online 28 August 2008.

Abstract

The freshwater nature reserve De Bruuk is an iron- and sulfur-rich minerotrophic peatland containing many iron seeps and forms a suitable habitat for iron and sulfur cycle bacteria. Analysis of 16S rRNA gene-based clone libraries showed a striking correlation of the bacterial population of samples from this freshwater ecosystem with the processes of iron reduction (genus Geobacter), iron oxidation (genera Leptothrix and Gallionella) and sulfur oxidation (genus Sulfuricurvum). Results from fluorescence in situ hybridization analyses with a probe specific for the beta-1 subgroup of Proteobacteria, to which the genera Leptothrix and Gallionella belong, and newly developed probes specific for the genera Geobacter and Sulfuricurvum, supported the clone library data. Molecular data suggested members of the epsilonproteobacterial genus Sulfuricurvum as contributors to the oxidation of reduced sulfur compounds in the iron seeps of De Bruuk. In an evaluation of anaerobic dimethyl sulfide (DMS)-degrading activity of sediment, incubations with the electron acceptors sulfate, ferric iron and nitrate were performed. The fastest conversion of DMS was observed with nitrate. Further, a DMS-oxidizing, nitrate-reducing enrichment culture was established with sediment material from De Bruuk. This culture was dominated by dimorphic, prosthecate bacteria, and the 16S rRNA gene sequence obtained from this enrichment was closely affiliated with Hyphomicrobium facile, which indicates that the Hyphomicrobium species are capable of both aerobic and nitrate-driven DMS degradation.