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Brief Communication
Nature Structural & Molecular Biology  11, 676 - 677 (2004)
Published online: 20 June 2004; | doi:10.1038/nsmb790

Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange

Jason Lanman1, 5, TuKiet T Lam2, 3, Mark R Emmett2, 3, Alan G Marshall2, 3, Michael Sakalian4 & Peter E Prevelige Jr1

1  Department of Microbiology, University of Alabama at Birmingham, Birmingham Alabama 35294, USA.

2  Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306, USA.

3  National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida 32310, USA.

4  Department of Microbiology and Immunology, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73190, USA.

5  Present address: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Correspondence should be addressed to Peter E Prevelige Jr prevelige@uab.edu
To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.


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Nature Structural & Molecular Biology
ISSN: 1545-9993
EISSN: 1545-9985
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