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Nature 418, 387-391 (25 July 2002) | doi:10.1038/nature00935;
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Assistant Editor – Nature Immunology
- Nature Publishing Group
- New York, NY United States
John Innes Centre Project Leader in Plant or Microbial Sciences
- University of East Anglia
- Norwich, NR4 7TJ, UK
Functional profiling of the Saccharomyces cerevisiae genome
Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed |[lsquo]|molecular bar codes|[rsquo]| uniquely identify each strain, enabling their growth to be analysed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays. We show that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment. Less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal growth in four of the tested conditions. Our results validate the yeast gene-deletion collection as a valuable resource for functional genomics.
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Abstract
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