Nature 368, 466 - 469 (31 March 1994); doi:10.1038/368466a0

An RNA polymerase II holoenzyme responsive to activators

Anthony J. Koleske & Richard A. Young

Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA

RNA POLYMERASE II requires multiple general transcription factors to initiate site-specific transcription^1–3. These proteins can assemble in an ordered fashion onto promoter DNA in vitro ^2–8, and such ordered assembly may occur in vivo (Fig. la). Some general transcription factors can interact with RNA polymerase II in the absence of DNA^3,9–15, however, suggesting that RNA polymerase II may also assemble into a multi-component complex containing a subset of initiation factors before binding to promoter DNA (Fig. Ib). Here we present evidence from the yeast Saccharo-myces cerevisiae for such an RNA polymerase II holoenzyme, a multi-subunit complex containing roughly equimolar amounts of RNA polymerase II, a subset of general transcription factors, and SRB regulatory proteins. Transcription by this holoenzyme is stimulated by the activator protein GAL4-VP16, a feature not observed with purified RNA polymerase II and general transcription factors alone. We propose that the holoenzyme is a form of RNA polymerase II readily recruited to promoters in vivo.

References

1.	Sawadogo, M. & Sentenac, A. A. Rev. Biochem. 59, 711−754 (1990). | Article | ChemPort |
2.	Zawel, L. & Reinberg, D. Curr. Opin. Cell Biol. 4, 488−495 (1992). | Article | PubMed | ChemPort |
3.	Conaway, R. C. & Conaway, J. W. A. Rev. Biochem. 62, 161−190 (1993). | Article | ChemPort |
4.	Davison, B. L., Egly, J. M., Mulvihill, E. R. & Chambon, P. Nature 301, 680−686 (1983). | Article | PubMed | ISI | ChemPort |
5.	Fire, A., Samuels, M. & Sharp, P. A. J. biol. Chem. 259, 2509−2516 (1984). | PubMed | ISI | ChemPort |
6.	Van Dyke, M. W., Roeder, R. G. & Sawadogo, M. Science 241, 1335−1338 (1988). | PubMed | ChemPort |
7.	Buratowski, S., Hahn, S., Guarente, L. & Sharp, P. A. Cell 56, 549−561 (1989). | Article | PubMed | ISI | ChemPort |
8.	Flores, O., Lu, H. & Reinberg, D. J. biol. Chem. 267, 2786−2793 (1992). | PubMed | ISI | ChemPort |
9.	Sopta, M., Carthew, R. W. & Greenblatt, J. J. biol. Chem. 260, 10353−10360 (1985). | PubMed | ChemPort |
10.	Price, D. H., Sluder, A. E. & Greenleaf, A. L. Molec. cell. Biol. 9, 1465−1475 (1989). | PubMed | ChemPort |
11.	Flores, O., Ha, I. & Reinberg, D. J. biol. Chem. 265, 5629−5634 (1990). | PubMed | ChemPort |
12.	Kitajima, S. et al. Nucleic Acids Res. 18, 4843−4849 (1990). | PubMed | ChemPort |
13.	Gerard, M. et al. J. biol. Chem. 266, 20940−20945 (1991). | PubMed | ISI | ChemPort |
14.	Henry, N. L., Sayre, M. H. & Kornberg, R. D. J. biol. Chem. 267, 23388−23392 (1992). | PubMed | ChemPort |
15.	Tschochner, H., Sayre, M. H., Flanagan, P. M., Feaver, W. J. & Kornberg, R. D. Proc. natn. Acad. Sci. U.S.A. 89, 11292−11296 (1992). | ChemPort |
16.	Thompson, C. M., Koleske, A. J., Chao, D. M. & Young, R. A. Cell 73, 1367−1375 (1993).
17.	Koleske, A. J., Buratowski, S., Nonet, M. & Young, R. A. Cell 69, 883−894 (1992). | Article | PubMed | ISI | ChemPort |
18.	Sayre, M. H., Tschochner, H. & Kornberg, R. D. J. biol. Chem. 267, 23383−23387 (1992). | PubMed | ISI | ChemPort |
19.	Sayre, M. H., Tschochner, H. & Kornberg, R. D. J. biol. Chem. 267, 23376−23382 (1992). | PubMed | ISI | ChemPort |
20.	Pinto, I., Ware, D. E. & Hampsey, M. Cell 68, 977−988 (1992). | Article | PubMed | ISI | ChemPort |
21.	Gileadi, O., Feaver, W. J. & Kornberg, R. D. Science 267, 1389−1391 (1992).
22.	Parvin, J. D. & Sharp, P. A. Cell 73, 533−540 (1993). | Article | PubMed | ISI | ChemPort |
23.	Ranish, J. A., Lane, W. S. & Hahn, S. Science 255, 1127−1132 (1992). | PubMed | ISI | ChemPort |
24.	Usheva, A. et al. Cell 69, 871−881 (1993).
25.	Flanagan, P. M., Kelleher, R. J., Sayre, M. H., Tschochner, H. & Kornberg, R. D. Nature 350, 436−438 (1991). | Article | PubMed | ISI | ChemPort |
26.	Flanagan, P. M., Kelleher, R. J. III, Tschochner, H., Sayre, M. H. & Kornberg, R. D. Proc. natn. Acad. Sci. U.S.A. 89, 7659−7663 (1992). | ChemPort |
27.	Payne, J. M., Laybourn, P. J. & Dahmus, M. E. J. biol. Chem. 264, 19621−19629 (1989). | PubMed | ISI | ChemPort |
28.	Laybourn, P. J. & Dahmus, M. E. J. biol. Chem. 265, 13165−13173 (1990). | PubMed | ISI | ChemPort |
29.	Lue, N. F., Flanagan, P. M., Sugimoto, K. & Kornberg, R. D. Science 246, 661−664 (1989). | PubMed | ISI | ChemPort |
30.	Liao, S-M., Taylor, I. C. A., Kingston, R. E. & Young, R. A. Genes Dev. 5, 2431−2440 (1991). | PubMed | ISI | ChemPort |