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The EMBO Journal (2001) 20, 5207–5218, doi:10.1093/emboj/20.18.5207 Figure 1 Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactions Cindy L. White, Robert K. Suto and Karolin Luger

Figure 1 Secondary and tertiary structure of the yeast nucleosome core particle. (A) Sequence alignment of X.laevis (top line) and S.cerevisiae histone proteins (bottom line). Amino acid differences are colored in magenta. Intervals of 10 amino acids for X.laevis (black circles) and S.cerevisiae (magenta circles) are indicated. The -helices and loops located within the structured regions are labeled, and the flexible histone tails are indicated by dashed lines. (B) The crystal structure of the yeast nucleosome core particle, viewed down the superhelical axis. Histone chains are colored yellow for H2A, red for H2B, blue for H3 and green for H4. The DNA is shown in turquoise. -helices and the location of the N- and C-terminal tails are shown. The position of the molecular dyad axis is indicated (). (C) Side view of the yeast nucleosome core particle, obtained by rotation of 90° around the axis of non-crystallographic symmetry, with part of the DNA removed for clarity. The arrow denotes the location of the L1 loop. (D and E) Amino acid differences in the yeast octamer [as shown in (A)] are colored according to the histone coloring scheme in (B). The conserved amino acids and DNA are shown in gray. Only 73 bp of the DNA and associated proteins are shown. (D) The solvent-exposed surface view of one half of the nucleosome is shown, while (E) shows the same half of the nucleosome viewed from the interior surface between the two gyres of the DNA supercoil.

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