Abstract
OBJECTIVE:
Oral treatment with oleoyl-estrone induces the loss of body fat and improvement of insulin resistance. Since cholesterol levels are deeply affected by oleoyl-estrone, we investigated here whether short-term treatment affected cholesterol turnover and overall metabolite changes.
DESIGN:
Wistar female rats received a single oral dose of 10 μmol/kg oleoyl-estrone in 0.2 ml of sunflower oil. Groups of animals were killed at timed intervals and blood samples were taken. In a second experiment series, rats had implanted carotid and jugular cannulas and were given a single gavage of oleoyl-estrone. These rats were used for the measurement of the cholesterol turnover rate.
MEASUREMENTS:
Body weight change and food intake: Glucose, total and HDL-cholesterol, triacylglycerols, 3-hydroxybutyrate, nonesterified fatty acids, insulin, HOMA score in the rats of the first series. Cholesterol: Cholesterol pool changes and cholesterol turnover rates in the rats of the second series.
RESULTS:
OE induced early effects, decreasing food intake, cholesterol and HDL-cholesterol levels, and increasing insulin sensitivity (HOMA score). OE also increased cholesteryl-ester turnover, and decreased circulating total cholesterol, especially esterified cholesterol pools.
CONCLUSIONS:
The role of early changes in insulin sensitivity induced by oral OE cannot explain per se the deep changes in cholesterol handling, essentially a consequence of accelerated lipoprotein turnover. However, the increase in cholesteryl-ester turnover observed with OE treatment may be, at least in part, a consequence of the decrease in insulin resistance. The compounded effect of increased insulin sensitivity and accelerated lipoprotein turnover may help explain the early and marked hypocholesterolaemic effects of OE.
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Acknowledgements
Funding by grants 01/1300 and 01/1309 from the Fondo de Investigaciones Sanitarias from the Government of Spain is gratefully acknowledged.
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Cabot, C., Salas, A., Ferrer-Lorente, R. et al. Short-term oral oleoyl-estrone treatment increases plasma cholesterol turnover in the rat. Int J Obes 29, 534–539 (2005). https://doi.org/10.1038/sj.ijo.0802898
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DOI: https://doi.org/10.1038/sj.ijo.0802898
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