Immunology and Cell Biology

FIGURE 3

FROM:

Axon growth and guidance genes identify T-dependent germinal centre B cells

Di Yu, Matthew C Cook, Dong-Mi Shin, Diego G Silva, Jennifer Marshall, Kai-Michael Toellner, Wendy L Havran, Pico Caroni, Michael P Cooke, Herbert C Morse, Ian CM MacLennan, Christopher C Goodnow and Carola G Vinuesa

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Figure 3.

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Expression of neurite-regulating genes by microarray and quantitative RT-PCR. (a) Signal value of selected genes by microarray analysis after normalization (y axis). (b) Quantitative RT-PCR in sorted resting and GC B cells from mice immunized by SRBC. The mRNA copy numbers were normalized by beta-actin. The fold increase of GC B cells to resting B cells is shown (each dot represents an independent experiment and grey bars represent mean values). TI4, TI GC group; TD4, TD D4 GC group; TD10, TD D10 GC group. RT, reverse transcription; GC, germinal centre; SRBC, sheep red blood cells; TI, T-independent.

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