Immunology and Cell Biology

FIGURE 1

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Mycobacterium tuberculosis secretory proteins CFP-10, ESAT-6 and the CFP10:ESAT6 complex inhibit lipopolysaccharide-induced NF-kappaB transactivation by downregulation of reactive oxidative species (ROS) production

Niladri Ganguly, Pham H Giang, Chitra Gupta, Sandip K Basu, Imran Siddiqui, Dinakar M Salunke and Pawan Sharma

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Figure 1.

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The circular dichroism spectra of CFP-10 (A), ESAT-6 (B) and the 1:1 CFP10:ESAT6 complex (C) measured in a Jasco J 500 polarimeter. The CD spectra were collected from protein samples dissolved in buffer 25 mM NaH2PO4, 100 mM NaCl, pH 6.5. Spectra were recorded from 190 to 250 nm at a scan speed of 50 nm min-1, each spectrum representing an average of 10 accumulations. During acquisition of spectra, the samples were maintained at 18 °C in a 0.1 mm path length cell.

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