FIGURE 2

Progressor skin tumours are infiltrated by a significantly greater percentage of macrophages while regressor tumours are populated by dendritic cells. (a) The first column of dot plots show the level of isotype staining on whole tumour cell preparations compared to the level of CD45 and CD11c staining (second column). Four-colour flow cytometry identified CD11c⁺CD45⁺ DC (R1) and CD11c^-CD45⁺ non-DC inflammatory cell infiltrates (R2). The third column of dot plots show the level of isotype staining for CD11b and MHC II on CD45⁺ cells compared to the expression of CD11b and MHC II on DC (R1; fourth column) and non-DC inflammatory cells (R2; last column of dot plots). TAM were identified as CD11c^-CD45⁺CD11b⁺ (last column in (a)). Dot plots are representative of four separate experiments. (b) The percentage of CD11c⁺CD45⁺ CD11b⁺ DC or CD11c^-CD45⁺CD11b⁺ TAM from four separate experiments were pooled. Mean+s.e.m. shown. ^* P<0.05. DC, dendritic cells; TAM, tumour-associated macrophages.