TABLE 1

FROM:

Isolation of myeloid and plasmacytoid dendritic cells from human bronchoalveolar lavage fluid

Maria Tsoumakidou, Nikolaos Tzanakis, Heleni A Papadaki, Heleni Koutala and Nikolaos M Siafakas

Table 1. mAbs used for flow cytometry

Specificity	Clone	Isotype	Conjugate	Source
mAbs used for evaluation of DC purity
BDCA-1	AD5-8E7	mIgG2a	PE	Miltenyi Biotec, Bergisch Gladbach, Germany
BDCA-2	AC144	mIgG1	FITC	Miltenyi Biotec
BDCA-3	AD5-14412	mIgG1	PE	Miltenyi Biotec
CD3	UCTHT1	mIgG1	FITC	Beckman Coulter Immunotech, Marseille, France
CD14	TÜK4	mIgG2a	PE-Cy5	Miltenyi Biotec
CD14	RMO52	mIgG2a	FITC	Beckman Coulter Immunotech
CD15	80H5	mIgM	PE	Beckman Coulter Immunotech
CD16	3G8	mIgG1	FITC	Beckman Coulter Immunotech
CD19	LT19	mIgG1	PE-Cy5	Miltenyi Biotec
CD19	J4.119	mIgG1	PE-Cy5	Beckman Coulter Immunotech
CD33	D3HL60	mIgG1	PE-Cy5	Beckman Coulter Immunotech
CD45	J.33	mIgG1	PE-Cy5	Beckman Coulter Immunotech
CD56	N901	mIgG1	PE	Beckman Coulter Immunotech
CD85k (ILT3)	ZM3.8	mIgG1	PE	Beckman Coulter Immunotech
CD123	107D2	mIgG1	PE-Cy5	Beckman Coulter Immunotech
HLA-DR	Immu-357	mIgG1	PE	Beckman Coulter Immunotech
mAbs used for evaluation of naive T-cell purity
CD3	UCTHT1	mIgG1	FITC	Beckman Coulter Immunotech
CD45RA	ALB11	mIgG1	PE	Beckman Coulter Immunotech
mAbs used for intracellular cytokine detection
IFN-	45.15	mIgG1	PE	Beckman Coulter Immunotech
IL-4	4D9	mIgG1	PE	Beckman Coulter Immunotech
IL-10	25209.1	mIgG1	PE	BD Pharmingen, San Diego, CA, USA
Controls
Control 1	679.1Mc7	mIgG1	FITC	Beckman Coulter Immunotech
Control 2	679.1Mc7	mIgG1	PE	Beckman Coulter Immunotech
Control 3	679.1Mc7	mIgG1	PE-Cy5	Beckman Coulter Immunotech
Control 4	543.10	mIgG2a	FITC	Miltenyi Biotec
Control 5	GC323	mIgM	PE	Beckman Coulter Immunotech

 DC, dendritic cell; PE, phycoerythrin; PE-Cy5, phycoerythrin-Cyanine 5.